Recently, sports medicine professionals have shown interest in using dynamic movement assessments to help identify biomechanical risk factors for musculoskeletal injury. Thus the purpose of this study was to propose two movements (single leg step down and single leg lateral hop) that could predict injury and determine if these proposed movements elicited muscle activation of the hamstrings and gluteals. Surface electromyography was employed and muscle activations of the hamstrings and gluteus medius muscles were classified as strong during both the single leg step down (SLSD) and single leg lateral hop (SLLH). Both the hamstrings and gluteus medius muscles are associated with musculoskeletal injury. The SLSD and SLLH cause significantly high muscle activation of both these muscle groups and should be considered for use in dynamic movement assessments.