Context: Reaction time is an indicator of cognitive function postconcussion and is an important measurement in return-to-play protocols. Current postconcussion assessments lack evidence regarding their reliability and do not simulate functional movement patterns and choice reactions that occur in sport performance. Objective: This study sought to establish the test–retest reliability of the FitLight Trainer in a nonconcussed, healthy population. Methods: Twenty-six healthy individuals from Bowling Green State University participated. Results: Good test–retest reliability was demonstrated for choice reaction time using the FitLight Trainer across the two testing sessions (intraclass correlation coefficient [2, 1] = .80). Conclusions: The FitLight Trainer provides reliable measures of reaction time in a healthy population.