All athletic trainers (ATs) must meet regulatory standards as outlined in state practice acts. While state practice acts are similar, some variations can lead to misunderstanding or unfamiliarity with appropriate scopes of practice. We aimed to describe ATs’ perceptions regarding athletic training scope of practice. Only 29.7% of respondents correctly identified state government as the agency that defines athletic training scope of practice and 51.7% agreed their respective state practice act limits the skills they can perform. To advocate for the profession, ATs must have a primary understanding of the laws and regulations that promote ATs to work at their fullest ability.