To test the effect of diet on the short-term lipid response to exercise, fourteen moderately trained (VO_2max: 50.2 ± 6.7 ml/kg/min), healthy men (mean age: 28 ± 4 years) were alternately fed a high fat (60±6.7% fat) and a high carbohydrate (63 ± 3.2% carbohydrate) isoenergetic diet for 2 weeks in a randomized crossover design. During the last 4 days of the treatments, fasting total cholesterol, triglyceride. HDL-cholesterol, and ${\text{HDL}}_3$-cholesterol were measured the day before, and again immediately, 24 hr. and 48 hr after exercise (4190 kJ, 70% ${\text{VO}}_{\mathrm{2max}}$). LDL-cholesterol and ${\text{HDL}}_2$-cholesterol were calculated. Lipid concentrations were adjusted for plasma volume changes after exercise. A 2 (diet) × 4 (time) ANOVA with repeated measures revealed no significant interaction between the diet and exercise treatments. Furthermore, diet alone did not influence lipid concentrations in these trained men. Exercise resulted in an increase in HDL-C (10.7%) and ${\text{HDL}}_3$-C (8.5%) concentrations and a concomitant fall in triglyceride (-25%) and total cholesterol (-3.5%). Thus, we conclude that diet composition does not affect the short-term changes in blood lipids and lipoproteins that accompany a single session of aerobic exercise in moderately trained men.