On two occasions, 8 male subjects completed a dehydration protocol, immediately followed by a 180-min rehydration protocol, then a subsequent exercise bout. During each dehydration session, subjects lost 3.1 ± 0.4% body weight (BW) following discontinuous exercise in the heat (40 °C, 33 % rh). During the first 30 min of rehydration, subjects ingested either 1.0-g glycerol · kg body weight−1 + 30% of the total rehydration water volume (GLY), or 30% of the total rehydration water volume without glycerol (CON). The five remaining ingestions (every 30 min) were equal to 14% of the remaining fluid volume and were identical in nature. Fluid volume ingested equaled fluid volume lost during dehydration. Following the 180 min rehydration period, subjects cycled (~50% V̇O2peak) in the heat (40 °C, 33% rh) until volitional exhaustion. Three observations were made: (a) Following glycerol-induced rehydration, time to volitional exhaustion was greater during the subsequent exercise bout in the heat (CON: 38.0 ± 2.0, GLY 42.8 ± 1.0 min, p < .05); (b) glycerol-induced rehydration significantly increased plasma volume restoration within 60 min and at the end of the 180-min rehydration period; and (c) total urine volume was lower and percent rehydration was greater following GLY, but neither was significantly different.