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The authors measured 24-h fluid-turnover (FTO) rate during 6 d of preseason training in U.S. college football players. Players, training (T, n = 9, full gear and contact drills) and reference (R, n = 4, conditioning without gear or contact), ingested a deuterium oxide (D2O) dose and provided urine samples every 24 h for analysis of D2O. During one ~2.3–h practice (wet-bulb globe temperature 24.6 °C), body-mass change, urine production, and voluntary fluid intake were measured to calculate gross sweat loss (GSL). Average FTO was 10.3 ± 2.2 L/d for T and 7.0 ± 1.0 L/d for R. GSL was 3.4 ± 1.5 L for T and 1.7 ± 1.3 for R (P > 0.05). By Day 6, body mass decreased significantly in T (–2.4 ± 1.3 kg, P < 0.05) but not in R (0.38 ± 0.95 kg). With preseason training under moderate environmental stress, football players had high FTO and sweat rates, which might have contributed to a loss of body mass during preseason football training.

Stofan, Osterberg, Horswill, Lacambra, and Murray are with the Gatorade Sports Science Institute, 617 W. Main Street, Barrington, IL 60010. Eichner is team internist, and Anderson, head athletic trainer, at the University of Oklahoma, Norman, OK.