The Effects of Plasmacytoid Dendritic Cell-Stimulative Lactic Acid Bacteria, Lactococcus lactis Strain Plasma, on Exercise-Induced Fatigue and Recovery via Immunomodulatory Action

in International Journal of Sport Nutrition and Exercise Metabolism

Click name to view affiliation

Takeshi KokuboKirin Company Limited

Search for other papers by Takeshi Kokubo in
Current site
Google Scholar
PubMed
Close
*
,
Yuta KomanoKirin Company Limited

Search for other papers by Yuta Komano in
Current site
Google Scholar
PubMed
Close
*
,
Ryohei TsujiKirin Company Limited

Search for other papers by Ryohei Tsuji in
Current site
Google Scholar
PubMed
Close
*
,
Daisuke FujiwaraKirin Company Limited

Search for other papers by Daisuke Fujiwara in
Current site
Google Scholar
PubMed
Close
*
,
Toshio FujiiKirin Company Limited

Search for other papers by Toshio Fujii in
Current site
Google Scholar
PubMed
Close
*
, and
Osamu KanauchiKirin Company Limited

Search for other papers by Osamu Kanauchi in
Current site
Google Scholar
PubMed
Close
*
Restricted access

The unique lactic acid bacteria, Lactococcus lactis strain plasma (LC-Plasma), stimulates plasmacytoid dendritic cells, which play an important role in viral infection. The authors previously reported that LC-Plasma reduced the number of days athletes experienced cold-like symptoms and fatigue feelings after high-intensity exercise training; however, the mechanism was unclear. In this study, the authors investigated the effect of LC-Plasma on recovery from physical damage after single exercise on a treadmill in BALB/c mice model. Oral administration of LC-Plasma (AIN-93G + 0.029% LC-Plasma) for 4 weeks significantly improved the locomotor reduction after treadmill exercise. This effect was not detected in mice receiving Lactobacillus rhamnosus GG, representative probiotics strain. LC-Plasma also improved voluntary locomotor activity after exercise. Blood and muscle sample analysis indicated that LC-Plasma affects plasmacytoid dendritic cell activation, which, in turn, attenuates muscle degenerative genes and the concentration of fatigue-controlled cytokine transforming growth factor-β.

The authors are with the Research Laboratories for Health Science & Food Technologies, Kirin Company Limited, Yokohama, Japan.

Kokubo (Takeshi_Kokubo@kirin.co.jp) is corresponding author.
Kokubo, Komano, and Tsuji contributed equally to this work.
  • Collapse
  • Expand
  • Arai, M., Yamazaki, H., Inoue, K., & Fushiki, T. (2002). Effects of intracranial injection of transforming growth factor-beta relevant to central fatigue on the waking electroencephalogram of rats: Comparison with effects of exercise. Progress in Neuropsychopharmacology & Biological Psychiatry, 26(2), 307312. PubMed ID: 11817509 doi:10.1016/S0278-5846(01)00272-X

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Berggren, A., Lazou Ahrén, I., Larsson, N., & Önning, G. (2011). Randomised, double-blind and placebo-controlled study using new probiotic lactobacilli for strengthening the body immune defence against viral infections. European Journal of Nutrition, 50(3), 203210. PubMed ID: 20803023 doi:10.1007/s00394-010-0127-6

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Bonsall, D.R., Kim, H., Tocci, C., Ndiaye, A., Petronzio, A., McKay-Corkum, G., … Harrington, M.E. (2015). Suppression of locomotor activity in female C57Bl/6J mice treated with interleukin-1β: investigating a method for the study of fatigue in laboratory animals. PLoS ONE, 10(10), 0140678. PubMed ID: 26469939 doi:10.1371/journal.pone.0140678

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Cervantes-Barragan, L., Lewis, K.L., Firner, S., Thiel, V., Hugues, S., Reith, W., … Reizis, B. (2012). Plasmacytoid dendritic cells control T-cell response to chronic viral infection. Proceedings of the National Academy of Sciences of the United States of America, 109(8), 30123017. PubMed ID: 22315415 doi:10.1073/pnas.1117359109

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Clark, A., & Mach, N. (2016). Exercise-induced stress behavior, gut-microbiota-brain axis and diet: A systematic review for athletes. Journal of the International Society of Sports Nutrition, 13, 43. PubMed ID: 27924137 doi:10.1186/s12970-016-0155-6

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Colbey, C., Cox, A.J., Pyne, D.B., Zhang, P., Cripps, A.W., & West, N.P. (2018). Upper respiratory symptoms, gut health and mucosal immunity in athletes. Sports Medicine, 48(Suppl. 1), 6577. PubMed ID: 29363055 doi:10.1007/s40279-017-0846-4

    • Crossref
    • Search Google Scholar
    • Export Citation
  • de Vrese, M., Winkler, P., Rautenberg, P., Harder, T., Noah, C., Laue, C., … Schrezenmeir, J. (2005). Effect of Lactobacillus gasseri PA 16/8, Bifidobacterium longum SP 07/3, B. bifidum MF 20/5 on common cold episodes: A double blind, randomized, controlled trial. Clinical Nutrition, 24(4), 481491. PubMed ID: 16054520 doi:10.1016/j.clnu.2005.02.006

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Fujitani, M., Matsumura, S., Masuda, D., Yamashita, S., Fushiki, T., & Inoue, K. (2014). CD36, but not GPR120, is required for efficient fatty acid utilization during endurance exercise. Bioscience, Biotechnology, and Biochemistry, 78(11), 18711878. PubMed ID: 25070011 doi:10.1080/09168451.2014.940835

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Harata, G., He, F., Hiruta, N., Kawase, M., Kubota, A., Hiramatsu, M., & Yausi, H. (2010). Intranasal administration of Lactobacillus Rhamnosus GG protects mice from H1N1 influenza virus infection by regulating respiratory immune responses. Letters in Applied Microbiology, 50(6), 597602. PubMed ID: 20438620 doi:10.1111/j.1472-765X.2010.02844.x

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Inoue, K., Yamazaki, H., Manabe, Y., Fukuda, C., Hanai, K., & Fushiki, T. (1999). Transforming growth factor-beta activated during exercise in brain depresses spontaneous motor activity of animals. Relevance to central fatigue. Brain Research, 846(2), 145153. PubMed ID: 10556630 doi:10.1016/S0006-8993(99)01966-6

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Jounai, K., Ikado, K., Sugimura, T., Ano, Y., Braun, J., & Fujiwara, D. (2012). Spherical lactic acid bacteria activate plasmacytoid dendritic cells immunomodulatory function via TLR9-dependent crosstalk with myeloid dendritic cells. PLoS One, 7(4), e32588. PubMed ID: 22505996 doi:10.1371/journal.pone.0032588

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Kanauchi, O., Andoh, A., AbuBakar, S., & Yamamoto, N. (2018). Probiotics and paraprobiotics in viral infection: Clinical application and effects on the innate and acquired immune systems. Current Pharmaceutical Design, 24(6), 710717. PubMed ID: 29345577 doi:10.2174/1381612824666180116163411

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Kashiwagi, I., Morita, R., Schichita, T., Komai, K., Saeki, K., Matsumoto, M., … Yoshimura, A. (2015). Smad2 and Smad3 inversely regulate TGF-beta autoinduction in clostridium butyricum-activated dendritic cells. Immunity, 43(1), 6579. PubMed ID: 26141582 doi:10.1016/j.immuni.2015.06.010

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Komano, Y., Shimada, K., Naito, H., Fukao, K., Ishihara, Y., Fujii, T., … Daida, H. (2018). Efficacy of heat-killed Lactococcus lactis JCM 5805 on immunity and fatigue during consecutive high intensity exercise in male athletes: A randomized, placebo-controlled, double-blinded trial. Journal of the International Society of Sports Nutrition, 15(1), 39. PubMed ID: 30071871 doi:10.1186/s12970-018-0244-9

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Lazar, V., Ditu, L.M., Pircalabioru, G.G., Gheorghe, I., Curutiu, C., Holban, A.M., … Chifiriuc, M.C. (2018). Aspects of gut microbiota and immune system interactions in infectious diseases, immunopathology, and cancer. Frontiers in Immunology, 9, 1830. PubMed ID: 30158926 doi:10.3389/fimmu.2018.01830

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Makelä, M.J., Puhakka, T., Ruuskanen, O., Leinonen, M., Saikku, P., Kimpimäki, M., … Arstila, P. (1998). Viruses and bacteria in the etiology of the common cold. Journal of Clinical Microbiology, 36(2), 539542. PubMed ID: 9466772

    • Search Google Scholar
    • Export Citation
  • Metz, J.P. (2003). Upper respiratory tract infections: Who plays, who sits? Current Sports Medicine Reports, 2(2), 8490. PubMed ID: 12831664 doi:10.1249/00149619-200304000-00007

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Miyazaki, K., Itoh, N., Yamamoto, S., Higo-Yamamoto, S., Nakakita, Y., Kaneda, H., … Oishi, K. (2014). Dietary heat-killed Lactobacillus Brevis SBC8803 promotes voluntary wheel-running and affects sleep rhythms in mice. Life Sciences, 111(1–2), 4752. PubMed ID: 25058921 doi:10.1016/j.lfs.2014.07.009

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Shibata, T., Kanayama, M., Haida, M., Fujimoto, S., Oroguchi, T., Sata, K., … Nishizaki, Y. (2016). Lactococcus lactis JCM5805 activates anti-viral immunity and reduces symptoms of common cold and influenza in healthy adults in a randomized controlled trial. Journal of Functional Foods, 24, 492500. doi:10.1016/j.jff.2016.03.035

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Siegal, F.P., Kadowaki, N., Shodell, M., Fitzgerald-Bocarsly, P.A., Shah, K., Ho, S., … Liu, Y.J. (1999). The nature of the principal type 1 interferon-producing cells in human blood. Science, 284(5421), 18351837. PubMed ID: 10364556 doi:10.1126/science.284.5421.1835

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Sugimura, T., Jounai, K., Ohshio, K., Suzuki, H., Kirisako, T., Sugihara, Y., & Fujiwara, D. (2018). Long-term administration of pDC-stimulative Lactococcus lactis strain decelerates senescence and prolongs the lifespan of mice. International Immunopharmacology, 58, 166172. PubMed ID: 29605632 doi:10.1016/j.intimp.2018.03.024

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Sugimura, T., Takahashi, H., Jounai, K., Ohshio, K., Kanayama, M., Tazumi, K., … Yamamoto, N. (2015). Effects of oral intake of plasmacytoid dendritic cells-stimulative lactic acid bacterial strain on pathogenesis of influenza-like illness and immunological response to influenza virus. British Journal of Nutrition, 114(5), 727733. PubMed ID: 26234407 doi:10.1017/S0007114515002408

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Travis, M.A., & Sheppard, D. (2014). TGF-β activation and function in immunity. Annual Review of Immunology, 32, 5182. PubMed ID: 24313777 doi:10.1146/annurev-immunol-032713-120257

    • Crossref
    • Search Google Scholar
    • Export Citation
  • VanderVeen, B.N., Fix, D.K., & Carson, J.A. (2017). Disrupted skeletal muscle mitochondrial dynamics, mitophagy, and biogenesis during cancer cachexia: A role for inflammation. Oxidative Medicine and Cellular Longevity, 2017, 3292087. PubMed ID: 28785374 doi:10.1155/2017/3292087

    • Crossref
    • Search Google Scholar
    • Export Citation
All Time Past Year Past 30 Days
Abstract Views 5698 2005 204
Full Text Views 134 18 0
PDF Downloads 127 37 0