Cannabidiol Does Not Impair Anabolic Signaling Following Eccentric Contractions in Rats

in International Journal of Sport Nutrition and Exercise Metabolism
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  • 1 University of California
  • 2 Charité-Universitätsmedizin Berlin
  • 3 University of California Davis
  • 4 VA Northern California Health Care System
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Cannabidiol (CBD) has proven clinical benefits in the treatment of seizures, inflammation, and pain. The recent legalization of CBD in many countries has caused increased interest in the drug as an over-the-counter treatment for athletes looking to improve recovery. However, no data on the effects of CBD on the adaptive response to exercise in muscle are available. To address this gap, we eccentrically loaded the tibialis anterior muscle of 14 rats, injected them with a vehicle (n = 7) or 100 mg/kg CBD (n = 7), and measured markers of injury, inflammation, anabolic signaling, and autophagy 18 hr later. Pro-inflammatory signaling through nuclear factor kappa B (NF-kB) (Ser536) increased with loading in both groups; however, the effect was significantly greater (36%) in the vehicle group (p < .05). Simultaneously, anabolic signaling through ribosomal protein S6 kinase beta-1 (S6K1) (Thr389) increased after eccentric contractions in both groups with no difference between vehicle and CBD (p = .66). The ribosomal protein S6 phosphorylation (240/244) increased with stimulation (p < .001) and tended to be higher in the CBD group (p = .09). The ubiquitin-binding protein p62 levels were not modulated by stimulation (p = .6), but they were 46% greater in the CBD compared with the vehicle group (p = .01). Although liver weight did not differ between the groups (p = .99) and levels of proteins associated with stress were similar, we did observe serious side effects in one animal. In conclusion, an acute dose of CBD decreased pro-inflammatory signaling in the tibialis anterior without blunting the anabolic response to exercise in rats. Future research should determine whether these effects translate to improved recovery without altering adaptation in humans.

Langer and Baar are with the Department of Physiology and Membrane Biology, University of California, Davis, Davis, CA, USA. Mossakowski is with the Charité—Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany. Mossakowski, Pathak, and Baar are with the Department of Neurobiology, Physiology and Behavior, University of California, Davis, Davis, CA, USA. Mascal is with the Department of Chemistry, University of California, Davis, Davis, CA, USA. Baar is also with the VA Northern California Health Care System, Mather, CA, USA.

Langer (htlanger@ucdavis.edu) is corresponding author.
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