Purpose: With female Australian football (AF) gaining popularity, understanding match demands is becoming increasingly important. The aim of this study was to compare running performances of rotated and whole-quarter state-level female AF players during match quarters. Methods: Twenty-two state-level female AF midfielders wore Global Positioning System units during 14 games to evaluate activity profiles. The Yo-Yo Intermittent Recovery Test Level 1 (Yo-Yo IR1) was used as a measure of high-intensity running ability. Data were categorized into whole quarter, rotation bout 1, and rotation bout 2 before being further divided into quartiles. Players were separated into high- or low-Yo-Yo IR1 groups using a median split based on their Yo-Yo IR1 performance. Short (4–6 min), moderate (6–12 min), and long (12–18 min) on-field bout activity profiles were compared with whole-quarter players. Results: High Yo-Yo IR1 performance allowed players to cover greater relative distances (ES = 0.57–0.88) and high-speed distances (ES = 0.57–0.86) during rotations. No differences were reported between Yo-Yo IR1 groups when players were required to play whole quarters (ES ≤ 0.26, likelihood ≤64%). Players who were on field for short to moderate durations exhibited greater activity profiles than whole-quarter players. Conclusions: Superior high-speed running ability results in a greater activity profile than for players who possess lower high-speed running ability. The findings also highlight the importance of short to moderate (4–12 min) rotation periods and may be used to increase high-intensity running performance within quarters in female AF players.