Purpose: To assess the reliability and sensitivity of commercially available inertial measurement units to measure physical activity in team handball. Method: Twenty-two handball players were instrumented with 2 inertial measurement units (OptimEye S5; Catapult Sports, Melbourne, Australia) taped together. They participated in either a laboratory assessment (n = 10) consisting of 7 team handball–specific tasks or field assessment (n = 12) conducted in 12 training sessions. Variables, including PlayerLoad™ and inertial movement analysis (IMA) magnitude and counts, were extracted from the manufacturers’ software. IMA counts were divided into intensity bands of low (1.5–2.5 m·s−1), medium (2.5–3.5 m·s−1), high (>3.5 m·s−1), medium/high (>2.5 m·s−1), and total (>1.5 m·s−1). Reliability between devices and sensitivity was established using coefficient of variation (CV) and smallest worthwhile difference (SWD). Results: Laboratory assessment: IMA magnitude showed a good reliability (CV = 3.1%) in well-controlled tasks. CV increased (4.4–6.7%) in more-complex tasks. Field assessment: Total IMA counts (CV = 1.8% and SWD = 2.5%), PlayerLoad (CV = 0.9% and SWD = 2.1%), and their associated variables (CV = 0.4–1.7%) showed a good reliability, well below the SWD. However, the CV of IMA increased when categorized into intensity bands (2.9–5.6%). Conclusion: The reliability of IMA counts was good when data were displayed as total, high, or medium/high counts. A good reliability for PlayerLoad and associated variables was evident. The CV of the previously mentioned variables was well below the SWD, suggesting that OptimEye’s inertial measurement unit and its software are sensitive for use in team handball.