Purpose: To (1) evaluate agreement between the PowerTap P1 (P1) pedals and the Lode Excalibur Sport cycle ergometer, (2) investigate the reliability of the P1 pedals between repeated testing sessions, and (3) compare the reliability and validity of the P1 pedals before (P1₀) and after (P1₁₀₀) ∼100 h of use. Methods: Ten participants completed four 5-min submaximal cycling bouts (100, 150, 200, and 250 W), a 2-min time trial, and two 10-s all-out sprints on 2 occasions. This protocol was repeated after 15 mo and ∼100 h of use. Results: Significant differences were seen between the P1₀ pedals and the Lode Excalibur Sport at 100 W (P = .006), 150 W (P = .006), 200 W (P = .001), and 250 W (P = .006) and during the all-out sprints (P = .020). After ∼100 h of use, the P1₁₀₀ pedals did not significantly differ from the Lode Excalibur Sport at 100 W (P = .799), 150 W (P = .183), 200 W (P = .289), and 250 W (P = .183), during the 2-min time trial (P = .583), or during the all-out sprints (P = .412). The coefficients of variation for the P1₀ and P1₁₀₀ ranged from 0.6% to 1.3% and 0.5% to 2.0%, respectively, during the submaximal cycling bouts. Conclusion: The P1 pedals provide valid data after ∼100 h of laboratory use. Furthermore, the pedals provide reliable data during submaximal cycling, even after prolonged use.