Sprint capacity is an important attribute for team-sport athletes, yet the most appropriate method to analyze it is unclear. Purpose: To examine the relationship between sprint workloads using relative versus absolute thresholds and lower-body soft-tissue and bone-stress injury incidence in professional Australian rules football. Methods: Fifty-three professional Australian rules football athletes’ noncontact soft-tissue and bone-stress lower-body injuries (N = 62) were recorded, and sprint workloads were quantified over ∼18 months using the global positioning system. Sprint volume (m) and exposures (n) were determined using 2 methods: absolute (>24.9 km·h−1) and relative (≥75%, ≥80%, ≥85%, ≥90%, ≥95% of maximal velocity). Relationships between threshold methods and injury incidence were assessed using logistic generalized additive models. Incidence rate ratios and model performances’ area under the curve were reported. Results: Mean (SD) maximal velocity for the group was 31.5 (1.4), range 28.6 to 34.9 km·h−1. In comparing relative and absolute thresholds, 75% maximal velocity equated to ~1.5 km·h−1 below the absolute speed threshold, while 80% and 85% maximal velocity were 0.1 and 1.7 km·h−1 above the absolute speed threshold, respectively. Model area under the curve ranged from 0.48 to 0.61. Very low and very high cumulative sprint loads ≥80% across a 4-week period, when measured relatively, resulted in higher incidence rate ratios (2.54–3.29), than absolute thresholds (1.18–1.58). Discussion: Monitoring sprinting volume relative to an athlete’s maximal velocity should be incorporated into athlete monitoring systems. Specifically, quantifying the distance covered at >80% maximal velocity will ensure greater accuracy in determining sprint workloads and associated injury risk.