Effect of Beetroot Juice Supplementation on Mood, Perceived Exertion, and Performance During a 30-Second Wingate Test

in International Journal of Sports Physiology and Performance
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Purpose: Dietary supplementation with inorganic nitrate (NO3) can enhance high-intensity exercise performance by improving skeletal muscle contractility and metabolism, but the extent to which this might be linked to altered psychophysiological processes is presently unclear. The purpose of this study was to assess the effects of NO3-rich beetroot juice (BJ) supplementation on profile of mood states, ratings of perceived exertion (RPE), and performance in a 30-second Wingate cycle test. Methods: In a double-blind, randomized, cross-over study, 15 subjects completed 2 laboratory sessions after ingesting NO3-rich or NO3-depleted (placebo) BJ. Participants initially completed the profile of mood states questionnaire. Subsequently, participants completed a warm-up followed by a 30-second all-out Wingate cycling test. After the Wingate test, participants immediately indicated the RPE of their leg muscles (RPEmuscular), cardiovascular system (RPEcardio), and general RPE (RPEgeneral). Results: Compared with the placebo condition, supplementation with BJ increased peak power output (Wpeak) (+4.4%, 11.5 [0.7] vs 11.1 [1.0] W·kg−1; P = .039) and lowered the time taken to reach Wpeak (7.3 [0.9] vs 8.7 [1.5] s; P = .002) during the Wingate test. The profile of mood states score linked to tension was increased prior to the Wingate test (4.8 [3.0] vs 3.4 [2.4]; P = .040), and RPEmuscular was lowered immediately following the Wingate test (17.7 [1.6] vs 18.3 [1.0]; P = .031), after BJ compared with placebo ingestion. Conclusions: Acute BJ supplementation improved pre-exercise tension, 30-second Wingate test performance, and lowered postexercise RPEmuscular.

Jodra and Veiga-Herreros are with the College of Health Sciences, Alfonso X El Sabio University, Madrid, Spain. Domínguez is with the Faculty of Health Sciences, Isabel I University, Burgos, Spain. Sánchez-Oliver is with the Faculty of Sports Sciences, Pablo de Olavide University, Seville, Spain; and the Human Motricity and Sports Performance Area, University of Seville, Seville, Spain. Bailey is with the School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, United Kingdom.

Domínguez (raul.dominguez@ui1.es) is corresponding author.
  • 1.

    Maughan RJ, Burke LM, Dvorak J, et al. IOC consensus statement: dietary supplements and the high-performance athlete. Br J Sports Med. 2018;52(7):439455. PubMed ID: 29540367 doi:10.1136/bjsports-2018-099027

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 2.

    Lundberg JO, Weitzberg E. NO-synthase independent NO generation in mammals. Biochem Biophys Res Commun. 2010;396(1):3945. PubMed ID: 20494108 doi:10.1016/j.bbrc.2010.02.136

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 3.

    Ferguson SK, Holdsworth CT, Wright JL, et al. Microvascular oxygen pressures in muscles comprised of different fiber types: impact of dietary nitrate supplementation. Nitric Oxide. 2015;48:3843. PubMed ID: 25280991 doi:10.1016/j.niox.2014.09.157

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 4.

    Bailey SJ, Fulford J, Vanhatalo A, et al. Dietary nitrate supplementation enhances muscle contractile efficiency during knee-extensor exercise in humans. J Appl Physiol. 2010;109(1):135148. PubMed ID: 20466802 doi:10.1152/japplphysiol.00046.2010

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 5.

    Haider G, Folland JP. Nitrate supplementation enhances the contractile properties of human skeletal muscle. Med Sci Sports Exerc. 2014;46(12):22342243. PubMed ID: 24681572 doi:10.1249/MSS.0000000000000351

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 6.

    Tanaka Y, Poole DC, Kano Y. pH homeostasis in contracting and recovering skeletal muscle: integrated function of the microcirculation with the interstitium and intramyocyte milieu. Curr Top Med Chem. 2016;16(24):26562663. PubMed ID: 27072709 doi:10.2174/1568026616666160413130221

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 7.

    Essén B. Glycogen depletion of different fibre types in human skeletal muscle during intermittent and continuous exercise. Acta Physiol Scand. 1978;103(4):446455. doi:10.1111/j.1748-1716.1978.tb06239.x

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 8.

    Domínguez R, Garnacho-Castaño MV, Cuenca E, et al. Effects of beetroot juice supplementation on a 30-s high-intensity inertial cycle ergometer test. Nutrients. 2017;9(12):1360. doi:10.3390/nu9121360

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 9.

    Marcora SM, Staiano W. The limit to exercise tolerance in humans: mind over muscle? Eur J Appl Physiol. 2010;109(4):763770. PubMed ID: 20221773 doi:10.1007/s00421-010-1418-6

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 10.

    Green P, Suls J. The effects of caffeine on ambulatory blood pressure, heart rate, and mood in coffee drinkers. J Behav Med. 1996;19(2):111128. PubMed ID: 9132505 doi:10.1007/BF01857602

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 11.

    Stafford LD, Rusted J, Yeomans MR. Caffeine, mood, and performance: a selective review. In: Smith BD, Gupta U, eds. Caffeine and Activation Theory : Effects on Health and Behavior. Boca Raton, FL: Taylor and Francis; 2007:284310. https://www.taylorfrancis.com/books/9781420006568/chapters/10.1201/9781420006568-20. Accessed January 17, 2019.

    • Search Google Scholar
    • Export Citation
  • 12.

    Mair RG, Onos KD, Hembrook JR. Cognitive activation by central thalamic stimulation: the Yerkes-Dodson law revisited. Dose Response. 2011;9(3):313331. PubMed ID: 22013395 doi:10.2203/dose-response.10-017.Mair

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 13.

    Pfeiffer KA, Pivarnik JM, Womack CJ, Reeves M, Malina RM. Reliability and validity of the Borg and OMNI RPE scales in adolescent females. Med Sci Sports Exerc. 2002;34(5):S278. https://insights.ovid.com/medicine-science-sports-exercise/mespex/2002/05/001/reliability-validity-borg-omni-rpe-scales/1559/00005768. Accessed January 17, 2019.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 14.

    Shannon OM, Barlow MJ, Duckworth L, et al. Dietary nitrate supplementation enhances short but not longer duration running time-trial performance. Eur J Appl Physiol. 2017;117(4):775785. PubMed ID: 28251402 doi:10.1007/s00421-017-3580-6

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 15.

    Murphy M, Eliot K, Heuertz RM, Weiss E. Whole beetroot consumption acutely improves running performance. J Acad Nutr Diet. 2012;112(4):548552. PubMed ID: 22709704 doi:10.1016/j.jand.2011.12.002

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 16.

    Presley TD, Morgan AR, Bechtold E, et al. Acute effect of a high nitrate diet on brain perfusion in older adults. Nitric Oxide. 2011;24(1):3442. PubMed ID: 20951824 doi:10.1016/j.niox.2010.10.002

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 17.

    Williamson JW, McColl R, Mathews D, Mitchell JH, Raven PB, Morgan WP. Hypnotic manipulation of effort sense during dynamic exercise: cardiovascular responses and brain activation. J Appl Physiol. 2001;90(4):13921399. PubMed ID: 11247939 doi:10.1152/jappl.2001.90.4.1392

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 18.

    Andersen JL, Aagaard P. Effects of strength training on muscle fiber types and size; consequences for athletes training for high-intensity sport. Scand J Med Sci Sports. 2010;20:3238. PubMed ID: 20840560 doi:10.1111/j.1600-0838.2010.01196.x

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 19.

    Fuentes I, Balaguer I, Meliá JL, García-Merita M. Forma abreviada del Perfil de Estado de Ánimo (POMS). V CongresoNacional de Psicología de La ActividadFísica y El Deporte; March 22–24, 1995. Valencia, Spain.

    • Export Citation
  • 20.

    Wylie LJ, Mohr M, Krustrup P, et al. Dietary nitrate supplementation improves team sport-specific intense intermittent exercise performance. Eur J Appl Physiol. 2013;113(7):16731684. PubMed ID: 23370859 doi:10.1007/s00421-013-2589-8

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 21.

    Shannon OM, Duckworth L, Barlow MJ, et al. Effects of dietary nitrate supplementation on physiological responses, cognitive function, and exercise performance at moderate and very-high simulated altitude. Front Physiol. 2017;8:401. PubMed ID: 28649204 doi:10.3389/fphys.2017.00401

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 22.

    Cuenca E, Jodra P, Pérez-López A, et al. Effects of beetroot juice supplementation on performance and fatigue in a 30-s all-out sprint exercise: a randomized, double-blind cross-over study. Nutrients. 2018;10(9):1222. doi:10.3390/nu10091222

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 23.

    Rimer EG, Peterson LR, Coggan AR, Martin JC. Increase in maximal cycling power with acute dietary nitrate supplementation. Int J Sports Physiol Perform. 2016;11(6):715720. PubMed ID: 26641379 doi:10.1123/ijspp.2015-0533

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 24.

    Jonvik KL, Nyakayiru J, Van Dijk JW, et al. Repeated-sprint performance and plasma responses following beetroot juice supplementation do not differ between recreational, competitive and elite sprint athletes. Eur J Sport Sci. 2018;18(4):524533. PubMed ID: 29412076 doi:10.1080/17461391.2018.1433722

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 25.

    Souissi M, Aloui A, Chtourou H, Aouicha HB, Atheymen R, Sahnoun Z. Caffeine ingestion does not affect afternoon muscle power and fatigue during the Wingate test in elite judo players. Biol Rhythm Res. 2015;46(2):291298. doi:10.1080/09291016.2014.994857

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 26.

    Stevens MJ, Lane AM, Terry PC. Mood profiling during Olympic qualifying judo competition: a case study testing transactional relationships. J Sports Sci Med. 2006;5(CSSI):143151. PubMed ID: 24357987.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 27.

    Thomas DD, Liu X, Kantrow SP, Lancaster JR, Jr. The biological lifetime of nitric oxide: implications for the perivascular dynamics of NO and O2. Proc Natl Acad Sci USA. 2001;98(1):355360. PubMed ID: 11134509 doi:10.1073/pnas.98.1.355

    • Crossref
    • Search Google Scholar
    • Export Citation
  • 28.

    Rooks CR, Thom NJ, McCully KK, Dishman RK. Effects of incremental exercise on cerebral oxygenation measured by near-infrared spectroscopy: a systematic review. Prog Neurobiol. 2010;92(2):134150. PubMed ID: 20542078 doi:10.1016/j.pneurobio.2010.06.002

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
  • 29.

    Amann M. Significance of Group III and IV muscle afferents for the endurance exercising human. Clin Exp Pharmacol Physiol. 2012;39(9):831835. PubMed ID: 22300329 doi:10.1111/j.1440-1681.2012.05681.x

    • Crossref
    • PubMed
    • Search Google Scholar
    • Export Citation
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