Background: Rest between training sessions can be short for athletes. In these situations, consuming carbohydrate (CHO) postexercise replenishes glycogen stores, which is important for recovery and subsequent performance. Purpose: This study tested whether CHO intake during a 2-hour rest between exercise bouts improved performance in the subsequent bout. Methods: In a randomized, single-blinded, crossover design, 10 recreationally active participants (23 [4] y, 70.8 [6.6] kg, 47.0 [5.4] mL·O₂·min⁻¹·kg·body·mass⁻¹) arrived at the lab postprandial and completed 2 exercise bouts separated by a 2-hour rest. Bouts included 5 × 4-minute intervals at ∼80% peak oxygen consumption separated by 2 minutes at ∼40% peak oxygen consumption and ended with an endurance trial to voluntary exhaustion at ∼90% peak oxygen consumption. During intervals 1 and 4 in each bout, expired gases were collected and O₂ deficit was estimated. Immediately following bout 1, either a CHO (1.2 g CHO·kg·body·mass⁻¹) or placebo solution was consumed. Results: Endurance trial duration decreased in bout 2 versus 1 in both conditions (P < .01) but was ∼35% longer in bout 2 with CHO versus placebo (interaction, P = .03; post hoc, P = .03). Oxygen uptake increased during interval 4 versus 1 in both bouts (P < .01) but was unaffected by CHO (P ≥ .58). O₂ deficit was unaffected by CHO (P = .93), bout, or interval (P ≥ .15). Perceived exertion was higher in bout 2 versus 1 (P < .001) and reduced in intervals 2 and 4 in CHO (P ≤ .01). Conclusions: When rest between training sessions is 2 hours, athletes may improve subsequent performance by consuming CHO during recovery.