Purpose: To evaluate the effect of drop jumps (DJs) on performance time and pacing in a field test (ie, 1000 m) commonly used to evaluate endurance runners and to evaluate running and jumping performance in male and female athletes separately. Methods: Twenty elite endurance runners (male, n = 10, 27.8 [7.0] y, 62.3 [5.2] kg; female, n = 10, 25.9 [5.3] y, 51.7 [4.1] kg) competing in middle- and long-distance events participated in this study. After determination of the box height associated with the best reactive strength index, athletes randomly performed a warm-up with or without the inclusion of 5 DJs with the highest reactive strength index prior to a 1000-m track test. Performance time and pacing (250-m splits) were determined. Countermovement-jump heights at different time points and blood lactate concentration after running tests were also recorded. Results: A “possible” faster 1000-m time (162.4 vs 165.3 s) with a “very likely” faster first split (38.8 vs 40.3 s) was observed in male athletes in the DJ condition. In contrast, female athletes showed a “possible” slower running time (186.8 vs 184.8 s) and a “likely” greater blood lactate concentration after the 1000-m test in the DJ condition. Male and female athletes presented greater countermovement-jump performances after warm-up and running tests in both conditions. Conclusions: The inclusion of 5 DJs with the height associated with the best reactive strength index induced a “possible” improvement in 1000-m performance time in elite male endurance runners. The current protocol should be avoided in female athletes.