Purpose: This study aimecd to investigate whether elite athletes could reach higher values of maximal oxygen uptake ($\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$) during a decremental exercise test in comparison with a traditional incremental test, as recently demonstrated in trained individuals. Methods: Nine male runners (age 25.8 [5.1] y, season best 10-km time 31:19 [1:50]) performed, on different days, 3 maximal uphill (5% grade) running exercise tests in fixed order: an incremental test (INC₁), a V-shape exercise test (where speed started at 0.5 km·h⁻¹ higher than the top stage finished during INC₁ and was slowly decreased during 5.5 min, when it was again increased in similar fashion to the INC tests), and a final incremental test (INC₂). Results: $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ during the V-shape exercise test was higher than during INC₁ (6.3% [3.0%], P = .01), although running speed was lower (16.6 [1.7] vs 17.9 [1.6] km·h⁻¹, P = .01). Performance was similar between INC₁ and INC₂, but $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ during INC₂ was higher than INC₁ (P < .001). During the V-shape exercise test, 5 participants reached the incremental part of the test, but $\dot{\mathrm{V}}{\mathrm{O}}_2$ did not increase ($\mathrm{\Delta }\dot{\mathrm{V}}{\mathrm{O}}_2=52\hspace{0.17em}[259]\mathrm{mL}·{\text{min}}^{-1}$, P = .67), despite higher running speed (approximately 1.1 km·h⁻¹, P < .01). Heart rate, pulmonary ventilation, breathing rate, and respiratory exchange ratio measured at $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ were not different between tests. Conclusion: A decremental exercise test of sufficient intensity can produce higher $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ than a traditional incremental test, even in elite athletes, and this is maintained during a subsequent incremental test.