Purpose: To examine the relationship between the maximal power output (MPO) in an individualized 7 × 2-minute incremental (INCR) test, average power in a 2k (W_2k) rowing ergometer test, and maximal oxygen uptake ($\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$) and to develop a regression equation to predict $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$. Methods: A total of 34 male club rowers (age 18–30 y) performed a 2k and an INCR test in a Concept2 rowing ergometer to determine and compare MPO, W_2k, and $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$. Results: No significant difference was found between $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ measured during INCR or 2k test (P = .73). A very high correlation coefficient (r = .96) was found between MPO and $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ and between W_2k and $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ (r = .93). Linear regression analyses were developed for predicting $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ from MPO: (1) $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ (mL·min⁻¹) = 11.49 × MPO + 810 and $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ from W_2k: (2) $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ = 10.96 × W_2k + 1168. Cross-validation analyses were performed using an independent sample of 14 rowers. There was no difference between the mean predicted $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ in the INCR test (4.41 L·min⁻¹) or the 2k test (4.39 L·min⁻¹) and the observed $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ (4.40 L·min⁻¹). Technical error of measurement was 3.1% and 3.6%, standard error of estimate was 0.136 and 0.157 mL·min⁻¹, and validation coefficients (r) were .95 and .94 using Equation (1) and (2), respectively. Conclusion: A prediction model only including MPO or W_2k explains 88% to 90% of the variability in $\dot{\mathrm{V}}{\mathrm{O}}_2\text{max}$ and is suggested for practical use in male club rowers.