Purpose: To identify the anaerobic threshold through the lactate threshold determined by Dmax and rating of perceived exertion (RPE) threshold by Dmax and to evaluate the agreement and correlation between lactate threshold determined by Dmax and RPE threshold by Dmax during an incremental test performed on the treadmill in long-distance runners. Methods: A total of 16 long-distance runners volunteered to participate in the study. Participants performed 2 treadmill incremental tests for the collection of blood lactate concentrations and RPE separated by a 48-hour interval. The incremental test started at 8 km·h−1, increasing by 1.2 km·h−1 every third minute until exhaustion. During each stage of the incremental test, there were pauses of 30 seconds for the collection of blood lactate concentration and RPE. Results: No significant difference was found between methods lactate threshold determined by Dmax and RPE threshold by Dmax methods (P = .664). In addition, a strong correlation (r = .91) and agreement through Bland–Altman plot analysis were found. Conclusions: The study demonstrated that it is possible to predict anaerobic threshold from the OMNI-walk/run scale curve through a single incremental test on the treadmill. However, further studies are needed to evaluate the reproducibility and objectivity of the OMNI-walk/run scale for anaerobic threshold determination.