Purpose: After anterior cruciate ligament reconstruction (ACL-R), knee muscle strength symmetry is used as part of the return-to-sport criteria. However, little is known about the changes in the force–velocity (F–V) relationship, which could affect athletic performance. This study investigated the F–V relationship of knee muscles at 4 and 8 months after ACL-R, using the 2-point method tested by isokinetic dynamometry. Methods: A total of 103 physically trained individuals (24.6 [9.3] y, 59.2% male) who underwent primary ACL-R were included. Demographic information and surgery characteristics were collected at 6 weeks postoperatively. Isokinetic knee flexors’ and extensors’ peak torques were measured at 60° and 240° per second in the concentric mode at 4 and 8 months postoperative. Peak torques and angular velocities were converted to force and linear velocity for calculating maximum isometric force (F0) and the slope of the regression line (F–V slope). Results: At 4 and 8 months postoperative, F0 was significantly lower and F–V slope was significantly less steep (less negative) on the operated leg compared with the nonoperated leg for knee extensors (P < .001) and flexors (P < .001–.002). The limb symmetry index calculated using F0 was lower than the limb symmetry indexes assessed at 60° and 240° per second, especially for knee flexors (P < .001). The use of patellar tendon grafts was associated with lower F0 and a less steep F–V slope compared with hamstring tendon grafts (P < .010). Conclusion: The isokinetic 2-point model assessing the F–V relationship provides additional and relevant insight for evaluating knee muscle strength after ACL-R.