Purpose: Submaximal sprinting allows for larger accumulated work to be reached before the onset of fatigue, compared with maximal efforts. The aim of this study was to investigate the effect of sprint running at 90% to 95% of maximal velocity (Vmax) on sprint performance. Methods: Recreationally active adults were randomly assigned into a control group (n = 12, 27 [5] y, 172 [9] cm, 72 [15] kg) and a training group (n = 14, 26 [4] y, 171 [9] cm, 69 [11] kg). Both groups completed pretesting and posttesting in form of a 30-m sprint separated by a 6-week period. The training group performed a weekly sprint-training session consisting of 30-m flying sprints at 90% to 95% of Vmax, while the control group performed no intervention training. Results: Significant improvements in the training group were observed for 10- (P = .003), 20- (P = .001), and 30-m sprint time (P = .002). These improvements were accompanied by higher step rate (P = .006) and theoretical Vmax (P = .007) and maximal power (P = .004). Significant between-groups differences were observed for 10- (P = .008), 20- (P < .001), and 30-m sprint time (P < .001), as well as for step rate (P = .015), theoretical Vmax (P = .016), and maximal power (P = .008). All within- and between-groups differences were in the range of trivial to small. Conclusion: Sprint running at 90% to 95% of Vmax can enhance 10- to 30-m sprint performance in recreationally active adults.