Purpose: Oxygen uptake kinetics (VO2kinetics) is a measure of an athlete’s capacity to respond to variations in energy demands. Faster VO2kinetics is associated with better performance in endurance sports, but optimal training methods to improve VO2kinetics remain unclear. This study compared the effects of 2 high-intensity interval-training (HIIT) programs on traditional rowing performance and VO2kinetics. Methods: Twelve highly trained rowers performed one of two 6-week HIIT protocols: either 3-minute repetitions at 90% (HIIT90; n = 5) of peak aerobic power (PAP) or 90-second repetitions at 100% (HIIT100; n = 7) of PAP. Before (PRE) and after (POST) the training intervention, they performed an incremental test to exhaustion to determine the individual lactate threshold, onset of blood lactate accumulation and PAP, and two 6-minute rest-to-exercise transitions to determine VO2kinetics. Results: No significant changes (P > .05) were observed for rowing ergometer power output at individual lactate threshold (HIIT90 PRE 255 [12], POST 264 [13]; HIIT100 247 [24], 266 [28] W), onset of blood lactate accumulation (279 [12], 291 [16]; 269 [23], 284 [32] W), or PAP (359 [13], 381 [15]; 351 [21], 363 [29] W) or for any parameters of VO2kinetics. No differences were observed between HIIT interventions. Conclusion: The HIIT interventions did not induce significant performance or VO2kinetics improvements, although mean power output at individual lactate threshold, onset of blood lactate accumulation, and PAP increased by 5.7%, 5.0%, and 4.5%, respectively. This suggests that the exact intensity and duration of HIIT sessions performed in the same intensity domain may be of lesser importance than other well-established influential factors (eg, training volume progression, training intensity distribution, altitude training) to develop aerobic qualities in endurance athletes.