Purpose: Most high-intensity bouts of exercise in volleyball consist of jumping activities, which are responsible for inducing muscle damage, high levels of fatigue, and muscle soreness. Therefore, the aim of the current study was to quantify and analyze the training loads, neuromuscular fatigue, and perceptual well-being of a 5-week in-season mesocycle carried out by a professional male volleyball team. Methods: Fifteen volleyball players (age 28.51 [5.39] y; height 193.19 [9.87] cm; body mass 88.46 [13.18] kg) participated in this study. Internal training load assessed through the rating of perceived exertion, external training load (ETL; evaluated using an inertial motion unit), countermovement jump (CMJ) height and peak power, and wellness questionnaire responses were obtained from all athletes. Results: Results indicated a progressive decrease of the internal training load during the week and by the undulatory pattern of the ETL during the microcycles. Moreover, training monotony increased across the microcycles and was negatively associated with CMJ peak power (r = −.681, P < .05). Finally, sleep quality (ρ = −.747, P < .01) and fatigue (ρ = −.789, P < .01) were negatively associated with weekly ETL. Conclusions: This study indicated that sleep quality and fatigue were negatively associated with weekly ETL. Therefore, decreases in weekly ETL might be needed to improve sleep quality and decrease fatigue in professional volleyball players. Plus, higher values of training monotony were associated with lower values of CMJ peak power. Consequently, avoiding training monotony might be important to improve jumping performance in professional volleyball athletes.