Background: Laboratory assessment of maximal oxygen uptake (${\dot{\mathrm{V}}\mathrm{O}}_2\text{max}$) is physically and mentally draining for the athlete and requires expensive laboratory equipment. Indirect measurement of ${\dot{\mathrm{V}}\mathrm{O}}_2\text{max}$ could provide a practical alternative to laboratory testing. Purpose: To examine the relationship between the maximal power output (MPO) in an individualized 7 × 2-minute incremental test (INCR-test) and ${\dot{\mathrm{V}}\mathrm{O}}_2\text{max}$ and to develop a regression equation to predict ${\dot{\mathrm{V}}\mathrm{O}}_2\text{max}$ from MPO in female rowers. Methods: Twenty female club and Olympic rowers (development group) performed the INCR-test on a Concept2 rowing ergometer to determine ${\dot{\mathrm{V}}\mathrm{O}}_2\text{max}$ and MPO. A linear regression analysis was used to develop a prediction of ${\dot{\mathrm{V}}\mathrm{O}}_2\text{max}$ from MPO. Cross-validation analysis of the prediction equation was performed using an independent sample of 10 female rowers (validation group). Results: A high correlation coefficient (r = .94) was found between MPO and ${\dot{\mathrm{V}}\mathrm{O}}_2\text{max}$. The following prediction equation was developed: ${\dot{\mathrm{V}}\mathrm{O}}_2\text{max}$ (mL·min⁻¹) = 9.58 × MPO (W) + 958. No difference was found between the mean predicted ${\dot{\mathrm{V}}\mathrm{O}}_2\text{max}$ in the INCR-test (3480 mL·min⁻¹) and the measured ${\dot{\mathrm{V}}\mathrm{O}}_2\text{max}$ (3530 mL·min⁻¹). The standard error of estimate was 162 mL·min⁻¹, and the percentage standard error of estimate was 4.6%. The prediction model only including MPO, determined during the INCR-test, explained 89% of the variability in ${\dot{\mathrm{V}}\mathrm{O}}_2\text{max}$. Conclusion: The INCR-test is a practical and accessible alternative to laboratory testing of ${\dot{\mathrm{V}}\mathrm{O}}_2\text{max}$.