Repeated-sprint ability is a significant factor in football performance. Notably, hamstring injuries in football players often occur during sprinting activities and fatigue-inducing conditions. Therefore, the aim of this study was to examine the impact of repeated-sprint training (RST) on repeated-sprint ability variables and sprint kinematics. Fourteen semiprofessional men’s soccer players performed 8 weeks of RST, consisting of 1 to 2 sets of 5 to 8 × 30 m repeated sprints separated by 20 to 30 seconds of recovery. Sprint performance was computed from running speed data, and a high-frequency camera (240 Hz) was used to study kinematic data. Paired samples t test and repeated-measures analysis of variance were conducted for each performance and kinematic variable, respectively. After the RST period, moderate to large improvements were observed for 0 to 20 m time, 0 to 30 m time, and 20 to 30 m time. All the repeated-sprint ability-related variables were significantly improved (P < .05). In addition, during fatigue conditions, a decrease in trunk flexion and kick-back mechanism and a reduced overstriding pattern was found after RST. The findings of this study suggest that incorporating RST may lead to improved sprint performance and promote a “safer” sprint pattern, particularly during periods of fatigue.