The calf raise test (CRT) is commonly used to assess triceps surae muscle-tendon unit function. Often, a metronome set to 60 beats/min (30 repetitions/min) is used to set the cadence of calf raise repetitions, but studies report using cadences ranging from 30 to 120 beats/min. We investigated the effect of cadence on CRT outcomes, accounting for the potential confounders of sex, age, body mass index, and physical activity. Thirty-six healthy individuals (50% female) performed single-leg calf raise repetitions to volitional exhaustion in 3 randomized cadence conditions, 7 days apart: 30, 60, and 120 beats/min. Repetitions, total vertical displacement, total work, peak height, and peak power were recorded using the validated Calf Raise application. Cadence significantly affected all CRT outcomes (P ≤ .008), except repetitions (P = .200). Post hoc analysis revealed 60 beats/min resulted in significantly greater total vertical displacement and work than 30 and 120 beats/min. Peak height was greater at 60 and 120 than 30 beats/min, and peak power was greater at 120 beats/min. Males generated greater work and peak power (P ≤ .001), whereas individuals with greater body mass index completed less repetitions (P = .008), achieved lower total vertical displacements (P = .003), and generated greater peak power (P = .005). CRT cadence is important to consider when interpreting CRT outcomes and comparing data between studies.