The authors developed and validated a “tailored” version of the Åstrand-Rhyming step test (tA-R) and a new equation for VO2max prediction in older adults (OA). Sixty subjects (age 68 ± 4 yr, 30 male, 30 female) performed their tA-R step test (5-min, 30-cm step, tailored stepping rate) and an incremental cycling test to exhaustion. VO2max was (a) predicted using the standard A-R equation (predictedVO2max), (b) predicted based on the authors’ new multiple linear equation (equationVO2max), and (c) directly measured by incremental cycling test (directVO2max). Agreement among values of VO2max was evaluated by Bland-Altman analysis. The predictedVO2max was not significantly different from the directVO2max, yet with relatively large imprecision. The equationVO2max allowed more precise as well as accurate predictions of VO2max compared with standard A-R prediction. The “tailored” version of the Åstrand-Rhyming step test and the new prediction equation appear suitable for a rapid (5-min), safe (submaximal), accurate, and precise VO2max prediction in healthy OA.