The development of the Older Persons’ Attitudes Toward Physical Activity and Exercise Questionnaire (OPAPAEQ) is reported. Working from first principles in psychometric test construction, the OPAPAEQ was developed and tested with 471 Canadians aged over 50 years. Through exploratory factor analysis and internal reliability analyses, four subscales were identified and labeled: Tension Release, Health Promotion, Vigorous Exercise, and Social Benefits. The four-factor structure was confirmed using confirmatory factor analysis. Small gender differences were noted on two factors, but no age differences were located. The OPAPAEQ is a tool suitable for further analysis of an age group identified as increasingly important in exercise and health promotion.