This study assessed the hormonal and psychological responses to a free-throw shooting competition in twelve NCAA Division I female collegiate basketball players. Salivary cortisol, alpha-amylase, and testosterone were collected before and after the competition, in addition to a self-reported measure of anxiety. Using nonparametric statistics, cortisol (Z = –3.06, p = .002) and testosterone (Z = –2.67, p = .008) levels were significantly higher precompetition compared with postcompetition. There were no statistically significant differences between winners and losers for anxiety or hormone responses. Concentration disruption (rho = .63, p = .03) and total competitive anxiety (rho = .68, p = .02) were positively correlated with precompetition cortisol. Concentration disruption also correlated positively with postcompetition cortisol (rho = .62 p = .03) and postcompetition testosterone (rho = .64, p = .03). Future studies are needed to examine the psychological and physiological stress responses of basketball players during different competition tasks.