Context: Identifying gender-specific differentiation in each sport type is significant. In this way, sport- and gender-specific gains can be predicted. Therefore, this study aimed to examine the effects of skiing on physical performance, pain, quality of life, and gender-based differentiation. Design: Crossover trial. Methods: Sixty-eight volunteers, between 18 and 25 years of age, with no history of severe trauma in the spine and extremities, participated in our study. The skiers group consisted of licensed athletes (17 males and 17 females) who did active skiing for at least the past 2 years, the control group consisted of nonathletic and age-matched participants with no skiing experience (17 males and 17 females). Step test, vertical jump test, Flamingo balance test, hand-grip strength, and back-leg-chest strength measurements were performed to evaluate physical performance. Visual Analog Scale, McGill Pain Questionnaire, and Oswestry Low Back Pain Disability Questionnaire were used to evaluate pain experience. Quality of life was evaluated with Short Form-36 (SF-36). Results: Compared to the sedentary controls, VAS-activity and Oswestry Disability Index scores were lower; and aerobic performance, balance, hand-grip strength, back-leg-chest strength, and quality of life (SF-36-general health, SF-36-vitality, SF-36-mental health, and SF-total score) were higher in skiers. Skiing was found to be effective in eliminating gender-based differentiation of the variables of hand-grip strength, pain, and quality of life; however, back-leg-chest strength and anaerobic performance variables were found to be ineffective in eliminating gender differentiation. Conclusions: Skiing allows that the individual increases physical performance and quality of life. It can also minimize gender-based differentiation of certain variables, such as muscular force, pain, and quality of life.