Interlimb Neuromuscular Responses During Fatiguing, Bilateral, Leg Extension Exercise at a Moderate Versus High Load

in Motor Control
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This study determined the load- and limb-dependent neuromuscular responses to fatiguing, bilateral, leg extension exercise performed at a moderate (50% one-repetition maximum [1RM]) and high load (80% 1RM). Twelve subjects completed 1RM testing for the bilateral leg extension, followed by repetitions to failure at 50% and 80% 1RM, on separate days. During all visits, the electromyographic (EMG) and mechanomyographic (MMG), amplitude (AMP) and mean power frequency (MPF) signals were recorded from the vastus lateralis of both limbs. There were no limb-dependent responses for any of the neuromuscular signals and no load-dependent responses for EMG AMP, MMG AMP, or MMG MPF (p = .301–.757), but there were main effects for time that indicated increases in EMG and MMG AMP and decreases in MMG MPF. There was a load-dependent decrease in EMG MPF over time (p = .032) that suggested variability in the mechanism responsible for metabolite accumulation at moderate versus high loads. These findings suggested that common drive from the central nervous system was used to modulate force during bilateral leg extension performed at moderate and high loads.

The authors are with the Department of Kinesiology and Healthy Promotion, University of Kentucky, Lexington, KY, USA.

Dinyer (taylor.dinyer@uky.edu) is corresponding author.
  • Abdi, H., & Williams, L.J. (2010). Newman–Keuls test and Tukey test. In N.J. Salkind (Ed.), Encyclopedia of research design. Thousand Oaks, CA: SAGE.

    • Search Google Scholar
    • Export Citation
  • Abe, T., Kumagai, K., & Brechue, W.F. (2000). Fascicle length of leg muscles is greater in sprinters than distance runners. Medicine & Science in Sports & Exercise, 32(6), 11251129. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Amman, M., Wan, H., Thurston, T.S., Georgescu, V.P., & Weavil, J.C. (2020). On the influence of group III/IV muscle afferent feedback on endurance exercise performance. Exercise and Sport Sciences Reviews, 48(41), 209216. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Anders, J.P.V., Smith, C.M., Keller, J.L., Hill, E.C., Housh, T.J., Schmidt, R.J., & Johnson, G.O. (2019). Inter- and intra-individual differences in EMG and MMG during maximal, bilateral, dynamic leg extensions. Sports, 7(7), 175. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Basmajian, J.V. (1979). Muscles alive: Their functions revealed by electromyography(4th ed.). Baltimore, MD: The Wilkins & Wilkins Company.

    • Search Google Scholar
    • Export Citation
  • Beck, T.W., & Housh, T.J. (2008). Use of electromyography in studying human movement. In Y. Hong& R. Bartlett (Eds.), Routledge handbook of biomechanics and human movement science (pp. 214227). New York, NY: Routledge.

    • Search Google Scholar
    • Export Citation
  • Beck, T.W., Housh, T.J., Cramer, J.T., Weir, J.P., Johnson, G.O., Coburn, J.W., Malek, M.H., & Mielke, M. (2005). Mechanomyographic amplitude and frequency responses during dynamic muscle actions: A comprehensive review. BioMedical Engineering OnLine, 4(1), 67. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Beck, T.W., Housh, T.J., Johnson, G.O., Cramer, J.T., Weir, J.P., Coburn, J.W., & Malek, M.H. (2007). Does the frequency content of the surface mechanomyographic signal reflect motor unit firing rates? A brief review. Journal of Electromyography and Kinesiology, 17, 113.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Bigland-Ritchie, B.R., Dawson, N.J., Johansson, R.S., Lippold, & O.C.J. (1986). Reflex origin for the slowing of motoneurone firing rates in fatigue of human voluntary contractions. Journal of Physiology, 379, 451459.

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Boonstra, T.W., Daffertshofer, A., van As, E., van der Vlugt, S., & Beek, P.J. (2007). Bilateral motor unit synchronization is functionally organized. Experimental Brain Research, 178(1), 7988. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Boonstra, T.W., Daffertschofer, A., van Ditshuizen, J.C., van den Heuvel, M.R.C., Hofman, C., Willingenburg, N.W., & Beek, P.J. (2008). Fatigue-related changes in motor-unit synchronization of quadriceps muscles within and across legs. Journal of Electromyography and Kinesiology, 18(5), 717731. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Cabibel, V., Hordacre, B., & Perrey, S. (2020). Implication of the ipsilateral motor network in unilateral voluntary muscle contraction: The cross-activation phenomenon. Journal of Neurophysiology, 123(5), 20902098. PubMed ID: 32319836 doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Contessa, P., De Luca, C.J., & Kline, J.C. (2016). The compensatory interaction between motor unit firing behavior and muscle force during fatigue. Journal of Neurophysiology, 116(4), 15791585. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Contessa, P., Letizi, J., De Luca, G., & Kline, J.C. (2018). Contribution from motor unit firing adaptations and muscle coactivation during fatigue. Journal of Neurophysiology, 119(6), 21862193. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Cresswell, A.G., & Ovendal, A.H. (2002). Muscle activation and torque development during maximal unilateral and bilateral isokinetic knee extensions. The Journal of Sports Medicine and Physical Fitness, 42(1), 1925. PubMed ID: 11832870

    • Search Google Scholar
    • Export Citation
  • De Luca, C.J., & Erim, Z. (1994). Common drive of motor units in regulation of muscle force. Trends in Neurosciences, 17(7), 299305. PubMed ID: 7524216 doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • De Luca, C.J., & Hostage, E.C. (2010). Relationship between firing rate and recruitment threshold of motoneurons in voluntary isometric contractions. Journal of Neurophysiology, 104(2), 10341046. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Dinyer, T.K., Byrd, M.T., Garver, M.J., Rickard, A.J., Miller, W.M., Burns, S., Clasey, J.L., & Bergstrom, H.C. (2019). Low-load vs. high-load resistance training to failure on one repetition maximum strength and body composition in untrained women. The Journal of Strength and Conditioning Research, 33, 17371744. PubMed ID: 31136545

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Dinyer, T.K., Byrd, M.T., Succi, P.J., & Bergstrom, H.C. (2020). The time course of changes in neuromuscular responses during the performance of leg extension repetitions to failure below and above critical resistance in women. The Journal of Strength and Conditioning Research. Advance online publication. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Enoka, R.M., & Stuart, D.G. (1992). Neurobiology of muscle fatigue. Journal of Applied Physiology, 72, 16311648. PubMed ID: 1601767

  • Henry, F.M., & Smith, L.E. (1961). Simultaneous vs. separate bilateral muscular contractions in relation to neural overflow theory and neuromotor specificity. Research Quarterly. American Association for Health, Physical Education and Recreation, 32(1), 4246. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Hermens, H.J., Freriks, B., Merletti, R., Stegeman, D., Blok, J., Rau, G., Disselhorst-Klug, C., & Hägg, G. (1999). European recommendations for surface electromyography. Enschede, The Netherlands: Roessingh Research and Development.

    • Search Google Scholar
    • Export Citation
  • Hu, X., Rymer, W.Z., & Suresh, N.L. (2014). Control of motor unit firing rate during step-like increases in voluntary force. Frontiers in Human Neuroscience, 8, 721. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Jenkins, N.D.M., Housh, T.J., Bergstrom, H.C., Cochrane, K.C., Hill, E.C., Smith, C.M., Johnson, G.O., Schmidt, R.J., & Cramer, J.T. (2015). Muscle activation during three sets to failure at 80 vs. 30 % 1RM resistance exercise. European Journal of Applied Physiology, 115, 23352347. PubMed ID: 26159316

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Kwatny, E., Thomas, D.H., & Kwatny, H.G. (1970). An application of signal processing techniques to the study of myoelectric signals. IEEE Transactions on Bio-Medical Engineering, 17(4), 303313. PubMed ID: 5518826 doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Malek, M.H., Coburn, J.W., Weir, J.P., Beck, T.W., & Housh, T.J. (2006). The effects of innervation zone on the electromyographic amplitude and mean power frequency during incremental cycling. Journal of Neuroscience Methods, 155(1), 126133. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Matkowski, B., Place, N., Martin, A., & Lepers, R. (2011). Neuromuscular fatigue differs following unilateral vs bilateral sustained submaximal contractions. Scandinavian Journal of Medicine & Science in Sports, 21(2), 268276. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Oda, S. (1997). Motor control for bilateral muscular contractions in humans. Japanese Journal of Physiology, 47, 487498. PubMed ID: 9538273

  • Sheppard, J.M., & Triplett, N.T. (2016). Program design for resistance training. In G.G. Haff& N.T. Triplett (Eds.), Essentials of strength training and conditioning (p. 453). Champaign, IL: Human Kinetics.

    • Search Google Scholar
    • Export Citation
  • Simoneau-Buessinger, E., Leteneur, S., Toumi, A., Dessurne, A., Gabrielli, F., Barbier, F., & Jakobi, J.M. (2015). Bilateral strength deficit is not neural in origin; rather due to dynamometer mechanical configuration. PLoS One, 10(12), e0145077. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
  • Smith, C.M., Housh, T.J., Jenkins, N.D.M., Hill, E.C., Cochrane, K.C., Miramonti, A.A., Schmidt, R.J., & Johnson, G.O. (2016). Combining regression and mean comparisons to identify the time course of changes in neuromuscular responses during the process of fatigue. Physiological Measurement, 37(11), 19932002. doi:

    • Crossref
    • Search Google Scholar
    • Export Citation
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