Peak cardiorespiratory responses, physical activity patterns, and the association of VO2peak and physical activity were examined in 16 elementary (ES) and 16 high school (HS) females. Peak responses were assessed during treadmill running, and physical activity patterns were examined over two 12-hour weekdays. Results indicated similar relative VO2peak responses between groups (ES: M = 46.8 ml · kg−1 · min−1;HS:M = 46.6 ml · kg−1 · min−1). No statistical differences (p ≤ .05) were noted when moderate to vigorous physical activity (MVPA) and vigorous physical activity (VPA) were compared. Also, a three-way (Group × HR level × Sustained minutes) ANOVA revealed no statistical differences. A median correlation (r = .27) was found from 8 independent correlations of habitual physical activity and VO2peak. ES and HS appeared similar in regard to VO2peak, accumulative and sustained MVPA and VPA. Low levels of sustained MVPA and VPA (≥ 10 min) were evident in both groups.