Alannah K.A. McKay, Peter Peeling, David B. Pyne, Nicolin Tee, Marijke Welveart, Ida A. Heikura, Avish P. Sharma, Jamie Whitfield, Megan L. Ross, Rachel P.L. van Swelm, Coby M. Laarakkers, and Louise M. Burke
This study implemented a 2-week high carbohydrate (CHO) diet intended to maximize CHO oxidation rates and examined the iron-regulatory response to a 26-km race walking effort. Twenty international-level, male race walkers were assigned to either a novel high CHO diet (MAX = 10 g/kg body mass CHO daily) inclusive of gut-training strategies, or a moderate CHO control diet (CON = 6 g/kg body mass CHO daily) for a 2-week training period. The athletes completed a 26-km race walking test protocol before and after the dietary intervention. Venous blood samples were collected pre-, post-, and 3 hr postexercise and measured for serum ferritin, interleukin-6, and hepcidin-25 concentrations. Similar decreases in serum ferritin (17–23%) occurred postintervention in MAX and CON. At the baseline, CON had a greater postexercise increase in interleukin-6 levels after 26 km of walking (20.1-fold, 95% CI [9.2, 35.7]) compared with MAX (10.2-fold, 95% CI [3.7, 18.7]). A similar finding was evident for hepcidin levels 3 hr postexercise (CON = 10.8-fold, 95% CI [4.8, 21.2]; MAX = 8.8-fold, 95% CI [3.9, 16.4]). Postintervention, there were no substantial differences in the interleukin-6 response (CON = 13.6-fold, 95% CI [9.2, 20.5]; MAX = 11.2-fold, 95% CI [6.5, 21.3]) or hepcidin levels (CON = 7.1-fold, 95% CI [2.1, 15.4]; MAX = 6.3-fold, 95% CI [1.8, 14.6]) between the dietary groups. Higher resting serum ferritin (p = .004) and hotter trial ambient temperatures (p = .014) were associated with greater hepcidin levels 3 hr postexercise. Very high CHO diets employed by endurance athletes to increase CHO oxidation have little impact on iron regulation in elite athletes. It appears that variations in serum ferritin concentration and ambient temperature, rather than dietary CHO, are associated with increased hepcidin concentrations 3 hr postexercise.
Henning T. Langer, Agata A. Mossakowski, Suraj Pathak, Mark Mascal, and Keith Baar
Cannabidiol (CBD) has proven clinical benefits in the treatment of seizures, inflammation, and pain. The recent legalization of CBD in many countries has caused increased interest in the drug as an over-the-counter treatment for athletes looking to improve recovery. However, no data on the effects of CBD on the adaptive response to exercise in muscle are available. To address this gap, we eccentrically loaded the tibialis anterior muscle of 14 rats, injected them with a vehicle (n = 7) or 100 mg/kg CBD (n = 7), and measured markers of injury, inflammation, anabolic signaling, and autophagy 18 hr later. Pro-inflammatory signaling through nuclear factor kappa B (NF-kB) (Ser536) increased with loading in both groups; however, the effect was significantly greater (36%) in the vehicle group (p < .05). Simultaneously, anabolic signaling through ribosomal protein S6 kinase beta-1 (S6K1) (Thr389) increased after eccentric contractions in both groups with no difference between vehicle and CBD (p = .66). The ribosomal protein S6 phosphorylation (240/244) increased with stimulation (p < .001) and tended to be higher in the CBD group (p = .09). The ubiquitin-binding protein p62 levels were not modulated by stimulation (p = .6), but they were 46% greater in the CBD compared with the vehicle group (p = .01). Although liver weight did not differ between the groups (p = .99) and levels of proteins associated with stress were similar, we did observe serious side effects in one animal. In conclusion, an acute dose of CBD decreased pro-inflammatory signaling in the tibialis anterior without blunting the anabolic response to exercise in rats. Future research should determine whether these effects translate to improved recovery without altering adaptation in humans.
Jacopo A. Vitale, Matteo Bonato, Lorenzo Petrucci, Giorgio Zucca, Antonio La Torre, and Giuseppe Banfi
Purpose: Little is known about the effect of sleep restriction (SR) on different domains of athletes’ physical performance. Therefore, the aim of this randomized, counterbalanced, and crossover study was to evaluate the effect of acute SR on sport-specific technical and athletic performance in male junior tennis players. Methods: Tennis players (N = 12; age 15.4 ± 2.6 y) were randomly allocated to either a sleep-restriction condition (SR, n = 6), where they experienced acute sleep restriction the night before the test session (≤5 h of sleep), or to a control condition (CON, n = 6), where they followed their habitual sleep–wake routines. Testing procedures included 20 left and right serves, 15 forehand and backhand crosscourt shots, and a repeated-sprint-ability test (RSA). The accuracy of serves and shots was considered for further analysis. One week later, players of SR joined CON, and players of CON experienced SR, and all test procedures were repeated. Results: Significant decrease in the accuracy of right (−17.5%, P = .010, effect size [ES] = 1.0, moderate) and left serve (−14.1%, P = .014, ES = 1.2, large), crosscourt backhand (−23.9%, P = .003, ES ≥ 2.0, very large), and forehand shot (−15.6%, P = .014, ES = 1.1, moderate) were observed in SR compared to CON, while RSA was similar in both conditions. Conclusion: Coaches and athletes at the team and individual level should be aware that 1 night of SR affects sport-specific but not athletic performance in tennis players.
Fernando G. Beltrami, Elena Roos, Marco von Ow, and Christina M. Spengler
Purpose: To compare the cardiorespiratory responses of a traditional session of high-intensity interval training session with that of a session of similar duration and average load, but with decreasing workload within each bout in cyclists and runners. Methods: A total of 15 cyclists (maximal oxygen uptake [
Naoya Takei, Jacky Soo, Hideo Hatta, and Olivier Girard
Background: Compared with normoxia, repeated short (5–10 s) sprints (>10 efforts) with incomplete recovery (≤30 s) in hypoxia likely cause substantial performance reduction accompanied by larger metabolic disturbances and magnitude of neuromuscular fatigue. However, the effects of hypoxia on performance of repeated long (30 s) “all-out” efforts with near complete recovery (4.5 min) and resulting metabolic and neuromuscular adjustments remain unclear. Purpose: The intention was to compare acute performance, metabolic, and neuromuscular responses across repeated Wingates between hypoxia and normoxia. Methods: On separate visits, 6 male participants performed 4 × 30-second Wingate efforts with 4.5-minute recovery in either hypoxia (fraction of inspired oxygen: 0.145) or normoxia. Responses to exercise (muscle and arterial oxygenation trends, heart rate, and blood lactate concentration) and the integrity of neuromuscular function in the knee extensors were assessed for each exercise bout. Results: Mean (P = .80) and peak (P = .92) power outputs, muscle oxygenation (P = .88), blood lactate concentration (P = .72), and perceptual responses (all Ps > .05) were not different between conditions. Arterial oxygen saturation was significantly lower, and heart rate higher, in hypoxia versus normoxia (P < .001). Maximal voluntary contraction force and peripheral fatigue indices (peak twitch force and doublets at low and high frequencies) decreased across efforts (all Ps < .001) irrespective of conditions (all Ps > .05). Conclusion: Despite heightened arterial hypoxemia and cardiovascular solicitation, hypoxic exposure during 4 repeated 30-second Wingate efforts had no effect on performance and accompanying metabolic and neuromuscular adjustments.
Peter Leo, James Spragg, Iñigo Mujika, Verena Menz, and Justin S. Lawley
Purpose: The aim of this study was to investigate changes in the power profile of U23 professional cyclists during a competitive season based on maximal mean power output (MMP) and derived critical power (CP) and work capacity above CP (W′) obtained during training and racing. Methods: A total of 13 highly trained U23 professional cyclists (age = 21.1 [1.2] y, maximum oxygen consumption = 73.8 [1.9] mL·kg–1·min–1) participated in this study. The cycling season was split into pre-season and in-season. In-season was divided into early-, mid-, and late-season periods. During pre-season, a CP test was completed to derive CPtest and W′test. In addition, 2-, 5-, and 12-minute MMP during in-season were used to derive CPfield and W′field. Results: There were no significant differences in absolute 2-, 5-, and 12-minute MMP, CPfield, and W′field between in-season periods. Due to changes in body mass, relative 12-minute MMP was higher in late-season compared with early-season (P = .025), whereas relative CPfield was higher in mid- and late-season (P = .031 and P = .038, respectively) compared with early-season. There was a strong correlation (r = .77–.83) between CPtest and CPfield in early- and mid-season but not late-season. Bland–Altman plots and standard error of estimates showed good agreement between CPtest and in-season CPfield but not between W′test and W′field. Conclusion: These findings reveal that the power profile remains unchanged throughout the in-season, except for relative 12-minute MMP and CPfield in late-season. One pre-season and one in-season CP test are recommended to evaluate in-season CPfield and W′field.
Patrick C. Maughan, Niall G. MacFarlane, and Paul A. Swinton
Purpose: To quantify and describe relationships between subjective and external measures of training load in professional youth soccer players. Methods: Data from differential ratings of perceived exertion (dRPE) and 7 measures of external training load were collected from 20 professional youth soccer players over a 46-week season. Relationships were described by repeated-measures correlation, principal component analysis, and factor analysis with oblimin rotation. Results: Significant positive (.44 ≤ r ≤ .99; P < .001) within-individual correlations were obtained across dRPE and all external training load measures. Correlation magnitudes were found to decrease when training load variables were expressed per minute. Principal component analysis provided 2 components, which described 83.3% of variance. The first component, which described 72.9% of variance, was heavily loaded by all measures of training load, while the second component, which described 10.4% of the variance, appeared to have a split between objective and subjective measures of volume and intensity. Exploratory factor analysis identified 4 theoretical factors, with correlations between factors ranging from .5 to .8. These factors could be theoretically described as objective volume, subjective volume, objective running, and objective high-intensity measures. Removing dRPE measures from the analysis altered the structure of the model, providing a 3-factor solution. Conclusions: The dRPE measures are significantly correlated with a range of external training load measures and with each other. More in-depth analysis showed that dRPE measures were highly related to each other, suggesting that, in this population, they would provide practitioners with similar information. Further analysis provided characteristic groupings of variables.