Prior to 1950, the field of exercise science was in its infancy. Exercise physiologists focused their research on understanding basic mechanisms of how the body responds to exercise and how to increase fitness. Most researchers and almost all research participants were male. Over the next two decades, and coinciding with the passage of Title IX, a few remarkable female exercise scientists emerged whose research and leadership had a profound effect on the field and directly influenced girls’ and women’s sports participation. This commentary presents an overview of the contributions and impact of several of these groundbreaking female exercise physiologists, Josephine Rathbone, Barbara Drinkwater, Priscilla Clarkson, Christine Wells, and Emily Haymes. We highlight their influence on the development of the field of exercise science and recognize their continued importance to women’s sport at the 50th Anniversary of Title IX.