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Effects of Whey Protein Supplementation Associated With Resistance Training on Muscular Strength, Hypertrophy, and Muscle Quality in Preconditioned Older Women

Paulo Sugihara Junior, Alex S. Ribeiro, Hellen C.G. Nabuco, Rodrigo R. Fernandes, Crisieli M. Tomeleri, Paolo M. Cunha, Danielle Venturini, Décio S. Barbosa, Brad J. Schoenfeld, and Edilson S. Cyrino

The purpose of this study was to investigate the effect of whey protein (WP) supplementation on muscular strength, hypertrophy, and muscular quality in older women preconditioned to resistance training (RT). In a randomized, double-blind, and placebo (PLA)-controlled design, 31 older women (67.4 ± 4.0 years, 62.0 ± 6.9 kg, 155.9 ± 5.7 cm, and 25.5 ± 2.4 kg/m2) received either 35 g of WP (n = 15) or 35 g of PLA (n = 16) over a 12-week study period while performing an RT program three times a week. Dietary intake, one-repetition maximum test, and skeletal muscle mass by dual-energy X-ray absorptiometry were assessed before and after the intervention period. Both groups showed significant (p < .05) improvements in skeletal muscle mass and total strength, and the WP group realized greater increases (p < .05) in these measures compared with PLA (skeletal muscle mass: WP = +4.8% vs. PLA = +2.3%; strength: WP = +8.7% vs. PLA = +4.9%). Muscular quality increased (p < .05) in both groups (WP = +2.9% vs. PLA = +1.5%) without statistical differences (p > .05) noted between conditions. We conclude that WP supplementation in combination with RT induces higher increases in both strength and hypertrophy in older women preconditioned to RT.

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Creatine Supplementation Does Not Influence the Ratio Between Intracellular Water and Skeletal Muscle Mass in Resistance-Trained Men

Alex S. Ribeiro, Ademar Avelar, Witalo Kassiano, João Pedro Nunes, Brad J. Schoenfeld, Andreo F. Aguiar, Michele C.C. Trindade, Analiza M. Silva, Luís B. Sardinha, and Edilson S. Cyrino

The authors aimed to compare the effects of creatine (Cr) supplementation combined with resistance training on skeletal muscle mass (SMM), total body water, intracellular water (ICW), and extracellular water (ECW) in resistance-trained men as well as to determine whether the SMM/ICW ratio changes in response to the use of this ergogenic aid. Twenty-seven resistance-trained men received either Cr (n = 14) or placebo (n = 13) over 8 weeks. During the same period, subjects performed two split resistance training routines four times per week. SMM was estimated from appendicular lean soft tissue assessed by dual-energy X-ray absorptiometry. Total body water, ICW, and ECW were determined by spectral bioelectrical impedance. Both groups showed improvements (p < .05) in SMM, total body water, and ICW, with greater values observed for the Cr group compared with placebo. ECW increased similarly in both groups (p < .05). The SMM/ICW ratio did not change in either group (p > .05), whereas the SMM/ECW ratio decreased only in the Cr group (p < .05). A positive correlation was observed (p < .05) between SMM and ICW changes (r = .71). The authors’ results suggest that the increase in muscle mass induced by Cr combined with resistance training occurs without alteration of the ratio of ICW to SMM in resistance-trained men.

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Effect of Resistance Training Systems on Oxidative Stress in Older Women

Alex S. Ribeiro, Rafael Deminice, Brad J. Schoenfeld, Crisieli M. Tomeleri, Camila S. Padilha, Danielle Venturini, Décio S. Barbosa, Luís B. Sardinha, and Edilson S. Cyrino

The purpose of this study was to investigate the effect of two different resistance training (RT) systems on oxidative stress biomarkers in older women. Fifty-nine older women (67.9 ± 5.0 years) were randomly assigned to one of three groups. Two training groups performed an 8 week RT program either in traditional (TD, n = 20) or a pyramid (PR, n = 20) system 3 times per week, or a control group (CG, n = 19). The TD program consisted of 3 sets of 8–12 RM with constant load for the 3 sets, whereas the PR training consisted of 3 sets of 12/10/8 RM with incremental loads for each set. As compared with the CG, both TD and PR achieved upregulation of the antioxidant system as evidenced by higher (p < .05) values of total radical-trapping antioxidant parameter plasma concentration after intervention (TD= 930.4 ± 160.0 µmolTrolox, PR= 977.8 ± 145.2 µmolTrolox, CG= 794.4 ± 130.2 µmolTrolox). For the protein oxidation adducts, TD and PR presented lower (p < .05) scores compared with CG (TD= 91.2 ± 25.0 µmol/L, PR= 93.0 ± 30.3 µmol/L, CG= 111.0 ± 20.4 µmol/L). However, there were no differences (p < .05) between trained groups in the antioxidant capacity markers and in the protein oxidation adducts markers. The results suggest that 8 weeks of progressive RT promotes an improvement in markers of oxidative stress in older women independent of the load-management RT system.

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Physical Activity and Sitting Time Are Specifically Associated With Multiple Chronic Diseases and Medicine Intake in Brazilian Older Adults

Alex S. Ribeiro, Luiz C. Pereira, Danilo R.P. Silva, Leandro dos Santos, Brad J. Schoenfeld, Denilson C. Teixeira, Edilson S. Cyrino, and Dartagnan P. Guedes

The purpose of the study was to clarify the independent association between sedentary behavior and physical activity with multiple chronic diseases and medicine intake in older individuals. Sedentary behavior and physical activity were measured by questionnaires. Diseases and medication use were self-reported. Poisson’s regression was adopted for main analysis, through crude and adjusted prevalence ratio and confidence interval of 95%. For men, sedentary time >4 hr/day presented a 76% higher prevalence of ≥2 chronic diseases, while physical inactivity increases the likelihood of using ≥2 medicines in 95%. For women, sedentary behavior >4 hr/day presented an 82% and 43% greater prevalence for ≥2 chronic diseases and the intake of ≥2 medicines, respectively. Sedentary behavior represents an independent associated factor of multiple chronic diseases in older men and women. In addition, inactivity for men and sedentarism for women are associated with the amount of medicine intake.

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Improvements in Phase Angle Are Related With Muscle Quality Index After Resistance Training in Older Women

João Pedro Nunes, Alex S. Ribeiro, Analiza M. Silva, Brad J. Schoenfeld, Leandro dos Santos, Paolo M. Cunha, Matheus A. Nascimento, Crisieli M. Tomeleri, Hellen C.G. Nabuco, Melissa Antunes, Letícia T. Cyrino, and Edilson S. Cyrino

The aim of this study was to analyze the association between muscle quality index (MQI) and phase angle (PhA) after a program of progressive resistance training (RT) in older women. Sixty-six older women with previous RT experience (68.8 ± 4.6 years, 156.6 ± 5.3 cm, 66.0 ± 13.0 kg, and 26.7 ± 4.6 kg/m2) underwent 12 weeks of RT (3 ×/week, eight exercises, and 10–15 repetition maximum). Anthropometry, muscular strength (one-repetition maximum tests), and body composition (dual-energy X-ray absorptiometry and spectral bioimpedance) were measured pre- and posttraining. There were observed significant increases for PhA, MQI, muscular strength, muscle mass, and reactance, whereas no significant changes in body fat and resistance were found. A significant correlation was observed between the RT-induced relative changes in PhA and MQI (r = .620). We conclude that improvements in MQI induced by RT are associated with increases in PhA. Therefore, PhA may be a valid tool to track changes in MQI after 12 weeks of RT in older women.