With the aim of supporting anxiety screening of student-athletes, this study examined the psychometric performance of the GAD-7 and GAD-2 for assessing anxiety and other clinical mental health concerns (depression, past-year and recent suicidality) in student-athletes. Data from intercollegiate varsity athletes (N = 7,584) were drawn from the Healthy Minds Study. Reliability estimates were good in the sample. Area under the curve values were excellent for anxiety and fair to good for depression and suicidality. Across all clinical indicators, a cutoff of 6 (GAD-7) and 2 (GAD-2), respectively, yielded the most balanced sensitivity and specificity rates. Both measures positively correlated with functional impairment, academic impact, and perceived mental health and negatively correlated with positive mental health. Overall, results supported the reliability, accuracy, and construct validity of the GAD-7 and GAD-2 in a national student-athlete sample. Discussion focuses on clinical implications and practical usage of the GAD-7 and GAD-2 with student-athletes.