This study investigated the relationship between organizational stressors in sport and athlete burnout and involved a cross-cultural comparison of English and Japanese soccer players. Ninety-eight male players completed the Athlete Burnout Questionnaire (Raedeke & Smith, 2001) to determine levels of perceived burnout. Based on data reported in previous research, and the thresholds developed by Hodge, Lonsdale, and Ng (2008), 22 of the players were identified as exhibiting higher levels of perceived burnout. Nine members of this subsample (4 English and 5 Japanese players) were subsequently interviewed to explore the relationship between their experiences of burnout and the organizational stressors they encountered. Results revealed multiple demands linked to the dimensions of athlete burnout and identified specific organizational-related issues that players associated with the incidence of burnout. Cultural differences between English and Japanese players in terms of the prevalence and organizational stressors associated with burnout were also identified, with the main differences being the relationship with senior teammates and the coaching style.