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David C. Nieman, Giuseppe Valacchi, Laurel M. Wentz, Francesca Ferrara, Alessandra Pecorelli, Brittany Woodby, Camila A. Sakaguchi, and Andrew Simonson

cell (PBMC) mRNA expression for cyclooxygenease-2 (COX-2). This measure increased significantly postexercise (time effect, p  < .001), but no significant interaction effect was measured ( p  = .322). Figure 4 —Peripheral blood mononuclear cell (PBMC) mRNA expression for glutathione peroxidase. This

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Mina Rasouli Mojez, Abbas Ali Gaeini, Siroos Choobineh, and Mohsen Sheykhlouvand

both cytosol and mitochondrial matrices including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX). Some small molecular weight and nonenzymatic antioxidants are also involved in the protection of the intracellular components against ROS along with the mentioned antioxidant

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Mohamed Romdhani, Nizar Souissi, Yassine Chaabouni, Kacem Mahdouani, Tarak Driss, Karim Chamari, and Omar Hammouda

and cellular damage. Free radicals production increases during the exercise because of the enhanced oxygen consumption and the activated phagocytic cells in the site of the damage. 26 Superoxide dismutase and glutathione peroxidase (GPx) are the first defense line against the exercise

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Rogério Bulhões Corvino, Débora da Luz Scheffer, Rafael Penteado dos Santos, Alexandra Latini, Anderson Souza Oliveira, and Fabrizio Caputo

, Instrumentation Laboratory, Lexington). Venous blood samples were taken to measure plasma glutathione (GSH), total thiol levels, and CK activity PRE, immediately POST, and 24 hours (for CK analyses only) after the exercise session. Samples were drawn from the anterior cubital vein using vacuum tubes (Vacuum

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Nathan A. Lewis, Ann Redgrave, Mark Homer, Richard Burden, Wendy Martinson, Brian Moore, and Charles R. Pedlar

suggestive of past (latent) EBV infection. Organic disease was excluded, and a diagnosis of UUPS was made. A number of investigations were undertaken: resting venous blood draws for the analysis of hydroperoxides (FORT), plasma antioxidant capacity (FORD), lutein, red blood cell glutathione (RBC GSH), α- and

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Fabricio de Souza, Luciano Acordi da Silva, Gisele Santinoni Ferreira, Márcia Mendonça Marcos de Souza, Franciane Bobinski, Juliete Palandi, Chaiana Esmeraldino Mendes Marcon, Daniel Fernandes Martins, Fabiana Schuelter-Trevisol, and Daisson José Trevisol

). The carbonyl content was determined spectrophotometrically at 370 nm using a molar absorption coefficient of 22,000 M −1 . Total glutathione levels (GSH) were measured in a reaction between DTNB and thiols that reached a maximum in 5 minutes. Absorbance was read at 412 nm after 10 minutes, and a

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Athanasios Kabasakalis, Stefanos Nikolaidis, George Tsalis, and Vassilis Mougios

upregulate biochemical markers such as lactate, glucose, cortisol, reduced glutathione (GSH), total antioxidant capacity, and 8-hydroxy-2′-deoxyguanosine. 11 Swimming SIT sets are considered relevant and effective in improving performance in competitive events ranging from 50 to 400 m, as they increase

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Sang-Ho Lee, Steven D. Scott, Elizabeth J. Pekas, Jeong-Gi Lee, and Song-Young Park

temperature. The incubated samples were stimulated by Ran-Cell total antioxidant control (Randox, Crumlin, United Kingdom) and then analyzed at 340 nm by plate reader. Plasma levels of glutathione peroxidase (GPx) were acquired by GPx Assay Kit (Cayman Chemical). Samples were incubated for 20 minutes at 37°C

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Rameswar Pal, Som Nath Singh, Kaushik Halder, Omveer Singh Tomer, Awadh Bihari Mishra, and Mantu Saha

Background:

This study was conducted to evaluate the effects of yogic practice on resting metabolism and redox status.

Methods:

The study was conducted on 64 physically trained male volunteers selected randomly at the Air Force Academy. The yoga group (n = 34) practiced yogasana, pranayama, and meditation for 3 months (February–May 2011) and the control group (n = 30) performed physical training. Antioxidant variables in blood samples along with physiological parameters were estimated before and after 3 months.

Results:

No significant difference was noted between baseline data of the control group and yoga group. Reduced glutathione, vitamin C, and vitamin E; the ratio of reduced to oxidized glutathione; and total antioxidant status were increased significantly following yogic practice. Activities of superoxide dismutase, glutathione S-transferase, and glutathione reductase were significantly increased, whereas activity of glutathione peroxidase was significantly decreased following yogic practice. Oxidized glutathione decreased significantly following yogic practice. A nonsignificant decrease of hydroperoxides, protein carbonyl, malondialdehyde, and blood sugar was noted in the yoga group. Carbon dioxide elimination and peripheral oxygen saturation increased significantly following yogic practice. No significant changes were observed in the control group following 3 months of physical training.

Conclusions:

Regular yogic practice can improve resting metabolism and redox status of the practitioner.

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Anna Skarpañska-Stejnborn, Lucia Pilaczynska-Szczesniak, Piotr Basta, Ewa Deskur-Smielecka, and Magorzata Horoszkiewicz-Hassan

High-intensity physical exercise decreases intracellular antioxidant potential. An enhanced antioxidant defense system is desirable in people subjected to exhaustive exercise. The aim of this study was to investigate the influence of supplementation with artichoke-leaf extract on parameters describing balance between oxidants and antioxidants in competitive rowers. This double-blinded study was carried out in 22 members of the Polish rowing team who were randomly assigned to a supplemented group (n = 12), receiving 1 gelatin capsule containing 400 mg of artichoke-leaf extract 3 times a day for 5 wk, or a placebo group (n = 10). At the beginning and end of the study participants performed a 2,000-m maximal test on a rowing ergometer. Before each exercise test, 1 min after the test completion, and after a 24-hr restitution period blood samples were taken from antecubital vein. The following redox parameters were assessed in red blood cells: superoxide dismutase activity, glutathione peroxidase activity, glutathione reductase activity, reduced glutathione levels, and thiobarbituric-acid-reactive-substances concentrations. Creatine kinase activity and total antioxidant capacity (TAC) were measured in plasma samples, lactate levels were determined in capillary blood samples, and serum lipid profiles were assessed. During restitution, plasma TAC was significantly higher (p < .05) in the supplemented group than in the placebo group. Serum total cholesterol levels at the end of the study were significantly (p < .05) lower in the supplemented group than in the placebo group. In conclusion, consuming artichoke-leaf extract, a natural vegetable preparation of high antioxidant potential, resulted in higher plasma TAC than placebo but did not limit oxidative damage to erythrocytes in competitive rowers subjected to strenuous training.