The purpose of this study was twofold: (a) to investigate the effect of the method of body segment parameter (BSP) estimation on the accuracy of the experimental simulation of a complex airborne movement; and (b) to assess the applicability of selected BSP estimation methods in the experimental simulation. It was hypothesized that different BSP estimation methods would provide different simulation results. A sensitivity analysis was performed to identify the BSP items and segments responsible for the inter-method differences in the simulation accuracy. The applicability of the estimation methods was assessed based on the simulation results and the number of anthropometric parameters required. Ten BSP estimation methods classified into 3 groups (4 cadaver-based, 4 gamma mass scanning-based, and 2 geometric) were employed in a series of experimental simulations based on 9 double-somersault-with-full-twist H-bar dismounts performed by 3 male college gymnasts. The simulated body orientation angles were compared with the corresponding observed orientation angles in computing the simulation errors. The inclination and twist simulation errors revealed significant (p < .05) differences among the BSP estimation groups and methods. It was concluded that: (a) the method of BSP estimation significantly affected the simulation accuracy, and more individualized BSP estimation methods generally provided more accurate simulation results; (b) the mass items, and the lower leg and thorax/ abdomen were more responsible for the intermethod differences in the simulation accuracy than other BSP items and segments, respectively; (c) the ratio methods and the simple regression methods were preferable in simulation of the somersaulting motion due to the fewer anthropometric parameters required; (d) the geometric models and the cadaver-based stepwise regression method were superior to the other methods in the simulation of the complex airborne motion with twist.