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Dietary Intake and Gastrointestinal Integrity in Runners Undertaking High-Intensity Exercise in the Heat

Naroa Etxebarria, Nicole A. Beard, Maree Gleeson, Alice Wallett, Warren A. McDonald, Kate L. Pumpa, and David B. Pyne

for biomarkers, which represent GI permeability, included lipopolysaccharide (LPS), lipopolysaccharide-binding protein, and intestinal fatty-acid-binding protein (i-FABP). All biomarkers were analyzed in duplicate, as previously described ( Wallett et al., 2020 ). Statistical Analysis The descriptive

Free access

The Impact of a Short-Term Ketogenic Low-Carbohydrate High-Fat Diet on Biomarkers of Intestinal Epithelial Integrity and Gastrointestinal Symptoms

Alannah K.A. McKay, Alice M. Wallett, Andrew J. McKune, Julien D. Périard, Philo Saunders, Jamie Whitfield, Nicolin Tee, Ida A. Heikura, Megan L.R. Ross, Avish P. Sharma, Ricardo J.S. Costa, and Louise M. Burke

concentrations of intestinal fatty acid-binding protein (I-FABP), lipopolysaccharide-binding protein (LBP), and soluble CD14 (sCD14) were measured in duplicate using commercially available enzyme-linked immunosorbent assay kits (HK406, HK315, and HK320, respectively; Hycult Biotech). The intraassay coefficient

Open access

Amino Acid-Based Beverage Interventions Ameliorate Exercise-Induced Gastrointestinal Syndrome in Response to Exertional-Heat Stress: The Heat Exertion Amino Acid Technology (HEAAT) Study

Ricardo J.S. Costa, Kayla Henningsen, Stephanie K. Gaskell, Rebekah Alcock, Alice Mika, Christopher Rauch, Samuel N. Cheuvront, Phil Blazy, and Robert Kenefick

50 μl that was used to determine plasma osmolality (P Osmol ), in duplicate (CV: 1.0%), by freezepoint osmometry (Osmomat 030, Gonotec). Plasma concentrations of cortisol (DKO001, DiaMetra), intestinal fatty acid protein (I-FABP; HK406, Hycult Biotech), soluble CD14 (sCD14; HK320, Hycult Biotech

Open access

Abstracts From the 6th Annual Congress on Medicine & Science in Ultra-Endurance Sports, October 11–13, 2019, Cape Town, South Africa

SILVA. Blood samples were collected pre- and post-exercise to determine I-FABP, cortisol, and cytokine profiles. Markers of physiological and thermoregulatory strain, and gastrointestinal symptoms (GIS) were measured throughout. Correlations and β-diversity analysis between amplicon sequence variants

Open access

Assessment of Exercise-Associated Gastrointestinal Perturbations in Research and Practical Settings: Methodological Concerns and Recommendations for Best Practice

Ricardo J.S. Costa, Pascale Young, Samantha K. Gill, Rhiannon M.J. Snipe, Stephanie Gaskell, Isabella Russo, and Louise M. Burke

standardize ( Pugh et al., 2019 ; Roberts et al., 2016 ; Zadow et al., 2021 ). Figure 2 —Exercise-associated disturbances to markers of gastrointestinal integrity, systemic responses, and GIS. Plasma (a) I-FABP, (b) sCD14, (c) LBP, (d) gram-negative bacterial endotoxin, (e) antiendotoxin IgM, (f

Open access

Abstracts From the December 2018 International Sports and Exercise Nutrition Conference in Newscastle upon Tyne

samples were collected pre- and post-exercise, and during recovery to determine plasma I-FABP and cortisol. Breath samples were collected every 30 min in recovery for 3 h to determine H 2 (malabsorption marker) responses. DEH resulted in higher heart rate (p = .038; 158 vs 152 bpm), RPE (p = .001; 15 vs

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Abstracts From the December 2017 International Sports and Exercise Nutrition Conference in Newscastle upon Tyne

. Immediately after exercise (or the equivalent time-period during the resting trial), participants underwent a 2-h oral glucose tolerance test (OGTT) with dual-stable isotope tracers to assess plasma glucose kinetics. Plasma concentrations of intestinal fatty acid binding protein (I-FABP) were assessed as a

Open access

Abstracts From the December 2019 International Sport + Exercise Nutrition Conference in Newscastle upon Tyne

lactulose to L -rhamnose (L:R) in serum. Plasma glutamine concentrations were measured at 2 h pre, post, and 1 h post heat stress and plasma intestinal fatty acid binding protein (I-FABP) concentrations were determined pre and post heat stress. Subjective heat illness symptoms were assessed throughout hot