Background: Jumps are important evaluation tools for muscle strength and power and for interlimb asymmetries. Different jump tests are well related to athletic performance, prediction of injury risk, and common motor gestures of several sports such as soccer. Low-cost mobile applications (apps) have gained popularity for this measure. The authors hypothesized that the My Jump 2 app would be a valid tool to assess drop-jump performance and interlimb asymmetry in soccer players. Methods: Eleven male soccer players took part in this study (18.2 [1.3] y, 69.9 [9.5] kg, 174 [6.6] cm). The athletes performed each test twice on a force plate (gold-standard method), while the jumps were recorded through the mobile app. Measures with the My Jump 2 app were applied by 2 evaluators, independently and in duplicate (interrater and intrarater reliability). The agreement analysis between both evaluations was done using an intraclass correlation coefficient and Bland–Altman plots. Results: Compared with the force platform, the app tested showed excellent reliability for the drop jump’s flight time and interlimb asymmetry (intraclass correlation coefficient > .98). For interlimb contact-time asymmetry, the values were 18.4 (9.9) and 19.1 (9.9) milliseconds for the My Jump 2 app and the force platform, respectively (P = .88). For flight-time asymmetries, the values were 389.7 (114.3) and 396.8 (112.5) milliseconds for the My Jump 2 app and the force platform, respectively (P = .88). Conclusion: The My Jump 2 app is a valid tool to assess drop-jump and interlimb asymmetry in soccer players.