Two popular methods of assessing lower body musculotendinous stiffness include the hopping and oscillation tests. The disparity and paucity of reliability data prompted this investigation into leg musculotendinous stiffness (Kleg) and ankle musculotendinous stiffness (Kank) measures. Kleg and Kank were assessed on three separate occasions in 20 female subjects. Kleg was determined using bilateral hopping procedures conducted at 2.2 Hz and 3.2 Hz frequencies. Kank was assessed by perturbation of the subject's ankle musculotendinous unit on an instrumented calf raise apparatus at 70% of maximum isometric force (MIF). Excellent reliability was produced for all Kleg measures between all days, whereas Kank exhibited acceptable reliability after one session of familiarization. No relationship was evident between Kleg and Kank. It was concluded that no familiarization session was required for Kleg at the test frequencies and conditions tested, whereas at least one familiarization session was needed to ensure the reliable assessment of Kank.