Context: Rounded shoulder posture is a common problem in the athletic population. Recently Kinesio tape has been utilized to improve balance, proprioception, and posture. However, the literature has been unable to provide definitive answers on the efficacy of Kinesio tape use. Objective: To determine the immediate effect of the limb rotational Kinesio tape application on the dynamic balance and proprioception of the shoulder measured by the Y-Balance Upper Quarter Test (YBT-UQ) in male collegiate athletes. Design: Cross-sectional. Setting: Sports medicine research laboratory.Participants: Nineteen healthy male collegiate National Association of Intercollegiate Athletics athletes (including rodeo, baseball, football, and soccer) with a mean age of 19.8 (1.4) years. Interventions: Subjects were randomized into Kinesio tape and non-Kinesio tape groups. The limb rotational Kinesio tape application was applied to the Kinesio tape group, while the non-Kinesio tape group received no intervention. Each group performed the YBT-UQ, which requires reaching in 3 directions in a push-up position, before and after the randomized intervention on a single day. Main Outcome Measures: The variables of interest included the maximum reach distance in each of the 3 directions and the composite score for both trials between the Kinesio tape and non-Kinesio tape groups. Each score was normalized against the subject’s limb length. Results: No statistically significant improvements in any YBT-UQ scores were observed following either the Kinesio tape or non-Kinesio tape intervention. Conclusions: Applying the limb rotational Kinesio tape technique did not improve immediate YBT-UQ scores in a male collegiate athletic population with rounded shoulder posture. The use of Kinesio tape to improve immediate closed kinetic chain function in male collegiate athletes with rounded shoulder posture cannot be supported.