This study aimed to provide further psychometric validation of the Sport Anxiety Scale-2 (SAS-2) by assessing the factor structure, invariance across gender, and convergent and divergent validity of the SAS-2 by correlating both related (i.e., anxiety sensitivity, brief fear of negative evaluation, intolerance of uncertainty, and negative affect) and unrelated constructs (i.e., positive affect, self-confidence). A total of 542 current and former competitive athletes completed a questionnaire through Amazon’s Mechanical Turk system. All data were collected via online survey. Participants were randomly assigned to an exploratory factor analysis (n = 271) and confirmatory factor analysis group (n = 271). Results indicated that both exploratory and confirmatory factor analyses supported the three-factor model of anxiety involving somatic anxiety, worry, and concentration disruption. Additionally, this study found the SAS-2 to be reliable, gender invariant, and have strong construct validity. Our findings extend the generalizability of the SAS-2 in more varied populations of athletic backgrounds.