Purpose: To examine the validity and reliability of a novel futsal special performance test (FSPT) as a measure of futsal performance and skills. Methods: Thirty-six futsal players with different levels of experience were recruited and divided into 2 groups (elite and nonelite). Players participated in 4 sessions (at least 7 d apart): (1) familiarization session, (2) anaerobic power (Wingate test), (3) FSPT trial 1, and (4) FSPT trial 2. The FSPT was carried out on a futsal court (wooden sprung floor) and skills such as dribbling, rotation, long and short passing, and shooting were examined. Content validity was assessed by 6 experienced futsal coaches and instructors. Results: There was a significant correlation between FSPT and various aspects of anaerobic power (r = .5–.91; P ≤ .001). Moreover, significant large correlations were observed between test and retest of FSPT (r = .77; 95% confidence interval [CI], .56–.98; P ≤ .001). All instructors and coaches confirmed the content validity. There was high interrater reliability of the FSPT (r = .89; 95% CI, .85–.93; P < .001). FSPT total time (P = .001), penalty time (P = .022), and performance time (P = .001) were superior in elite relative to nonelite players. Anaerobic power was greater in elite players (P < .001). Conclusion: The results support the use of the FSPT to assess futsal players’ performance in conjunction with skill and anaerobic fitness.