The aim of this study is to investigate the effect of material property changes in the spinal components on the resonant frequency characteristics of the human spine. Several investigations have reported the material property sensitivity of human spine under static loading conditions, but less research has been devoted to the material property sensitivity of spinal biomechanical characteristics under a vibration environment. A detailed three-dimensional finite element model of the human spine, T12– pelvis, was built and used to predict the influence of material property variation on the resonant frequencies of the human spine. The simulation results reveal that material properties of spinal components have obvious influences on the dynamic characteristics of the spine. The annulus ground substance is the dominant component affecting the vertical resonant frequencies of the spine. The percentage change of the resonant frequency relative to the basic condition was more than 20% if Young’s modulus of disc annulus is less than 1.5 MPa. The vertical resonant frequency may also decrease if Poisson’s ratio of nucleus pulposus of intervertebral disc decreases.