Drinking games (DGs) participation is prevalent among college-attending emerging adults. Research also suggests that student-athletes play DGs more frequently than non student-athletes, but what motivates student-athletes to participate in DGs is not well understood. Using data from a larger longitudinal study with Division III female athletes, we examined the test-retest reliability and minimal detectable change of the revised 7-factor Motives for Playing Drinking Games (MPDG) measure, and explored how its subscales were related to DGs behavior across two annual timepoints (n = 49). Results indicated that the MPDG shows adequate test-retest reliability over a one year period among student-athletes. Controlling for age and general alcohol consumption, conformity motives were positively associated with DG consumption at timepoint 1, whereas the DG motives of enhancement/thrills and boredom were positively related to DG consumption at timepoint 2. Implications for future research directions on motives for playing DGs and DGs behavior among student-athletes are discussed.