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Daniel J. Plews, Paul B. Laursen, and Martin Buchheit

Purpose:

Heart-rate variability (HRV) is a popular tool for monitoring autonomic nervous system status and training adaptation in athletes. It is believed that increases in HRV indicate effective training adaptation, but these are not always apparent in elite athletes.

Methods:

Resting HRV was recorded in 4 elite rowers (rowers A, B, C, and D) over the 7-wk period before their success at the 2015 World Rowing Championships. The natural logarithm of the square root of the mean sum of the squared differences (Ln rMSSD) between R–R intervals, Ln rMSSD:R-R ratio trends, and the Ln-rMSSD–to–R-R-interval relationship were assessed for each championship-winning rower.

Results:

The time course of change in Ln rMSSD was athlete-dependent, with stagnation and decreases apparent. However, there were consistent substantial reductions in the Ln rMSSD:R-R ratio: rower A, baseline toward wk 5 (–2.35 ± 1.94); rower B, baseline to wk 4 and 5 (–0.41 ± 0.48 and –0.64 ± 0.65, respectively); rower C, baseline to wk 4 (–0.58 ± 0.66); and rower D, baseline to wk 4, 5, and 6 (–1.15 ± 0.91, –0.81 ± 0.74, and –1.43 ± 0.69, respectively).

Conclusions:

Reductions in Ln rMSSD concurrent with reductions in the Ln rMSSD:R-R ratio are indicative of parasympathetic saturation. Consequently, 3 of 4 rowers displayed substantial increases in parasympathetic activity despite having decreases in Ln rMSSD. These results confirm that a combination of indices should be used to monitor cardiac autonomic activity.

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Ed Maunder, Paul B. Laursen, and Andrew E. Kilding

Purpose:

To compare the physiological and performance effects of ad libitum cold-fluid (CF) and ice-slurry (IS) ingestion on cycling time-trial (TT) performance in the heat.

Methods:

Seven well-trained male triathletes and cyclists completed 2 maximaleffort 40-km cycling TTs in hot (35°C) and humid (60% relative humidity) conditions. In randomized order, participants ingested CF or IS (initial temperatures 4°C and –1°C, respectively) ad libitum during exercise. At each 5-km interval, time elapsed, power output, rectal and skin temperature, heart rate, and perceptual measures were recorded. The actual CF and IS temperatures during the 40-km TT were determined post hoc.

Results:

Performance time (2.5% ± 2.6%, ES = 0.27) and mean power (–2.2% ± 3.2%, ES = –0.15) were likely worse in the IS trial. Differences in thermoregulatory and cardiovascular measures were largely unclear between trials, while feeling state was worse in the later stages of the IS trial (ES = –0.31 to –0.95). Fluid-ingestion volume was very likely lower in the IS trial (–29.7% ± 19.4%, ES = –0.97). The temperatures of CF and IS increased by 0.37°C/min and 0.02°C/min, respectively, over the mean TT duration.

Conclusions:

Ad libitum ingestion of CF resulted in improved 40-km cycling TT performance compared with IS. Participants chose greater fluid-ingestion rates in the CF trial than in the IS trial and had improved feeling state. These findings suggest that ad libitum CF ingestion is preferable to IS during cycling TTs under conditions of environmental heat stress.

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Gregory T. Levin, Paul B. Laursen, and Chris R. Abbiss

Purpose:

To assess the reliability of a 5-min-stage graded exercise test (GXT) and determine the association between physiological attributes and performance over stochastic cycling trials of varying distance.

Methods:

Twenty-eight well-trained male cyclists performed 2 GXTs and either a 30-km (n = 17) or a 100-km stochastic cycling time trial (n = 9). Stochastic cycling trials included periods of high-intensity efforts for durations of 250 m, 1 km, or 4 km depending on the test being performing.

Results:

Maximal physiological attributes were found to be extremely reliable (maximal oxygen uptake [VO2max]: coefficient of variation [CV] 3.0%, intraclass correlation coefficient [ICC] .911; peak power output [PPO]: CV 3.0%, ICC .913), but a greater variability was found in ventilatory thresholds and economy. All physiological variables measured during the GXT, except economy at 200 W, were correlated with 30-km cycling performance. Power output during the 250-m and 1-km efforts of the 30-km trial were correlated with VO2max, PPO, and the power output at the second ventilatory threshold (r = .58–.82). PPO was the only physiological attributed measured during the GXT to be correlated with performance during the 100-km cycling trial (r = .64).

Conclusions:

Many physiological variables from a reliable GXT were associated with performance over shorter (30-km) but not longer (100-km) stochastic cycling trials.

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Al Haddad Hani, Paul B. Laursen, Ahmaidi Said, and Buchheit Martin

Purpose:

To assess the effect of supramaximal intermittent exercise on long-term cardiac autonomic activity, inferred from heart rate variability (HRV).

Methods:

Eleven healthy males performed a series of two consecutive intermittent 15-s runs at 95% VIFT (i.e., speed reached at the end of the 30-15 Intermittent Fitness Test) interspersed with 15 s of active recovery at 45% VIFT until exhaustion. Beat-to-beat intervals were recorded during two consecutive nights (habituation night and 1st night) before, 10 min before and immediately after exercise, as well as 12 h (2nd night) and 36 h (3rd night) after supramaximal intermittent exercise. The HRV indices were calculated from the last 5 min of resting and recovery periods, and the first 10 min of the first estimated slow wave sleep period.

Results:

Immediate post-supramaximal exercise vagal-related HRV indices were significantly lower than immediate pre-supramaximal exercise values (P < .001). Most vagal-related indices were lower during the 2nd night compared with the 1st night (eg, mean RR intervals, P = .03). Compared with the 2nd night, vagal-related HRV indices were significantly higher during the 3rd night. Variables were not different between the 1st and 3rd nights; however, we noted a tendency (adjusted effect size, aES) for an increased normalized high-frequency component (P = .06 and aES = 0.70) and a tendency toward a decreased low-frequency component (P = .06 and aES = 0.74).

Conclusion:

Results confirm the strong influence of exercise intensity on short- and long-term post exercise heart rate variability recovery and might help explain the high efficiency of supramaximal training for enhancing indices of cardiorespiratory fitness.

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Jonathan Peake, Jeremiah J. Peiffer, Chris R. Abbiss, Kazunori Nosaka, Paul B. Laursen, and Katsuhiko Suzuki

Purpose:

Heat stress might attenuate the effects of carbohydrate on immunoendocrine responses to exercise by increasing endogenous glucose production and reducing the rate of exogenous carbohydrate oxidation. The authors compared the efficacy of carbohydrate consumption on immune responses to exercise in temperate vs. hot conditions.

Methods:

Ten male cyclists exercised on 2 separate occasions in temperate (18.1 ± 0.4 °C, 58% ± 8% relative humidity) and on another 2 occasions in hot conditions (32.2 ± 0.7 °C, 55% ± 2% relative humidity). On each occasion, the cyclists exercised in a fed state for 90 min at ~60% VO2max and then completed a 16.1-km time trial. Every 15 min during the first 90 min of exercise, they consumed 0.24 g/kg body mass of a carbohydrate or placebo gel.

Results:

Neutrophil counts increased during exercise in all trials (p < .05) and were significantly lower (40%, p = .006) after the carbohydrate than after the placebo trial in 32 °C. The concentrations of serum interleukin (IL)-6, IL-8, and IL-10 and plasma granulocyte-colony-stimulating factor, myeloperoxidase, and calprotectin also increased during exercise in all trials but did not differ significantly between the carbohydrate and placebo trials. Plasma norepinephrine concentration increased during exercise in all trials and was significantly higher (50%, p = .01) after the carbohydrate vs. the placebo trial in 32 °C.

Conclusion:

Carbohydrate ingestion attenuated neutrophil counts during exercise in hot conditions, whereas it had no effect on any other immune variables in either temperate or hot conditions.

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Joseph A. McQuillan, Deborah K. Dulson, Paul B. Laursen, and Andrew E. Kilding

We aimed to compare the effects of two different dosing durations of dietary nitrate (NO3 -) supplementation on 1 and 4 km cycling time-trial performance in highly trained cyclists. In a double-blind crossover-design, nine highly trained cyclists ingested 140ml of NO3 --rich beetroot juice containing ~8.0mmol [NO3 -], or placebo, for seven days. Participants completed a range of laboratory-based trials to quantify physiological and perceptual responses and cycling performance: time-trials on day 3 and 6 (4km) and on day 4 and 7 (1km) of the supplementation period. Relative to placebo, effects following 3- and 4-days of NO3 - supplementation were unclear for 4 (-0.8; 95% CL, ± 2.8%, p = .54) and likely harmful for 1km (-1.9; ± 2.5% CL, p = .17) time-trial mean power. Effects following 6- and 7-days of NO3 - supplementation resulted in unclear effects for 4 (0.1; ± 2.2% CL, p = .93) and 1km (-0.9; ± 2.6%CL, p = .51) time-trial mean power. Relative to placebo, effects for 40, 50, and 60% peak power output were unclear for economy at days 3 and 6 of NO3 - supplementation (p > .05). Dietary NO3 - supplementation appears to be detrimental to 1km time-trial performance in highly trained cyclists after 4-days. While, extending NO3 - dosing to ≥ 6-days reduced the magnitude of harm in both distances, overall performance in short duration cycling time-trials did not improve relative to placebo.

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Joseph A. McQuillan, Deborah K. Dulson, Paul B. Laursen, and Andrew E. Kilding

Purpose:

To determine the effect of dietary nitrate (NO3 ) supplementation on physiology and performance in well-trained cyclists after 6–8 d of NO3 supplementation.

Methods:

Eight competitive male cyclists (mean ± SD age 26 ± 8 y, body mass 76.7 ± 6.9 kg, VO2peak 63 ± 4 mL · kg–1 · min–1) participated in a double-blind, placebo-controlled, crossover-design study in which participants ingested 70 mL of beetroot juice containing ~4 mmol NO3 (NIT) or a NO3 -depleted placebo (PLA), each for 8 d. Replicating pretreatment measures, participants undertook an incremental ramp assessment to determine VO2peak and first (VT1) and second (VT2) ventilatory thresholds on d 6 (NIT6 and PLA6), moderate-intensity cycling economy on d 7 (NIT7 and PLA7), and a 4-km time trial (TT) on d 8 (NIT8 and PLA8).

Results:

Relative to PLA, 6 d of NIT supplementation produced unclear effects for VO2peak (mean ± 95% confidence limit: 1.8% ± 5.5%) and VT1 (3.7% ± 12.3%) and trivial effects for both VT2 (–1.0% ± 3.0%) and exercise economy on d 7 (–1.0% ± 1.6%). However, effects for TT performance time (–0.7% ± 0.9%) and power (2.4% ± 2.5%) on d 8 were likely beneficial.

Conclusions:

Despite mostly unclear outcomes for standard physiological determinants of performance, 8 d of NO3 supplementation resulted in likely beneficial improvements to 4-km TT performance in well-trained male endurance cyclists.

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Thomas Reeve, Ralph Gordon, Paul B. Laursen, Jason K.W. Lee, and Christopher J. Tyler

Purpose: To investigate the effects of short-term, high-intensity interval-training (HIIT) heat acclimation (HA). Methods: Male cyclists/triathletes were assigned into either an HA (n = 13) or a comparison (COMP, n = 10) group. HA completed 3 cycling heat stress tests (HSTs) to exhaustion (60% W max; HST1, pre-HA; HST2, post-HA; HST3, 7 d post-HA). HA consisted of 30-min bouts of HIIT cycling (6 min at 50% W max, then 12 × 1-min 100%-W max bouts with 1-min rests between bouts) on 5 consecutive days. COMP completed HST1 and HST2 only. HST and HA trials were conducted in 35°C/50% relative humidity. Cycling capacity and physiological and perceptual data were recorded. Results: Cycling capacity was impaired after HIIT HA (77.2 [34.2] min vs 56.2 [24.4] min, P = .03) and did not return to baseline after 7 d of no HA (59.2 [37.4] min). Capacity in HST1 and HST2 was similar in COMP (43.5 [8.3] min vs 46.8 [15.7] min, P = .54). HIIT HA lowered resting rectal (37.0°C [0.3°C] vs 36.8°C [0.2°C], P = .05) and body temperature (36.0°C [0.3°C] vs 35.8°C [0.3°C], P = .03) in HST2 compared with HST1 and lowered mean skin temperature (35.4°C [0.5°C] vs 35.1°C [0.3°C], P = .02) and perceived strain on day 5 compared with day 1 of HA. All other data were unaffected. Conclusions: Cycling capacity was impaired in the heat after 5 d of consecutive HIIT HA despite some heat adaptation. Based on data, this approach is not recommended for athletes preparing to compete in the heat; however, it is possible that it may be beneficial if a state of overreaching is avoided.

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Gareth N. Sandford, Sian V. Allen, Andrew E. Kilding, Angus Ross, and Paul B. Laursen

Purpose: In recent years (2011–2016), men’s 800-m championship running performances have required greater speed than previous eras (2000–2009). The “anaerobic speed reserve” (ASR) may be a key differentiator of this performance, but profiles of elite 800-m runners and their relationship to performance time have yet to be determined. Methods: The ASR—determined as the difference between maximal sprint speed (MSS) and predicted maximal aerobic speed (MAS)—of 19 elite 800- and 1500-m runners was assessed using 50-m sprint and 1500-m race performance times. Profiles of 3 athlete subgroups were examined using cluster analysis and the speed reserve ratio (SRR), defined as MSS/MAS. Results: For the same MAS, MSS and ASR showed very large negative (both r = −.74 ± .30, ±90% confidence limits; very likely) relationships with 800-m performance time. In contrast, for the same MSS, ASR and MAS had small negative relationships (both r = −.16 ± .54; possibly) with 800-m performance. ASR, 800-m personal best, and SRR best defined the 3 subgroups along a continuum of 800-m runners, with SRR values as follows: 400–800 m ≥ 1.58, 800 m ≤ 1.57 to ≥ 1.48, and 800–1500 m ≤ 1.47 to ≥ 1.36. Conclusion: MSS had the strongest relationship with 800-m performance, whereby for the same MSS, MAS and ASR showed only small relationships to differences in 800-m time. Furthermore, the findings support the coaching observation of three 800-m subgroups, with the SRR potentially representing a useful and practical tool for identifying an athlete’s 800-m profile. Future investigations should consider the SRR framework and its application for individualized training approaches in this event.

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Katherine T. Oberlin-Brown, Rodney Siegel, Andrew E. Kilding, and Paul B. Laursen

The oral presence of carbohydrate (CHO) and caffeine (CAF) may independently enhance exercise performance, but their influence on performance during prolonged exercise is less known.

Aim:

To determine the independent and combined effects of CHO and CAF administered in chewing gum during a cycling time trial (TT) after prolonged exercise.

Method:

Eleven male cyclists (32.2 ± 7.5 y, 74.3 ± 6.8 kg, 60.2 ± 4.0 mL · kg–1 · min1 V˙O2peak) performed 4 experimental trials consisting of 90-min constant-load cycling at 80% of their second ventilatory threshold (207 ± 30 W), followed immediately by a 20-km TT. Under double-blinded conditions, cyclists received placebo (PLA), CHO, CAF, or a combined CHO+CAF chewing gum at 0-, 5-, 10-, and 15-km points of the TT.

Results:

Overall TT performance was similar across experimental and PLA trials (%mean difference ± 90%CL 0.2% ± 2.0%, 0.4% ± 2.2%, 0.1% ± 1.8% for CHO, CAF, and CHO+CAF). Compared with PLA, mean power output tended to be higher in the first 2 quarters of the TT with CHO (1.6% ± 3.1% and 0.8% ± 2.0%) and was substantially improved in the last 2 quarters during CAF and CHO+CAF trials (4.2% ± 3.0% and 2.0% ± 1.8%). There were no differences in average heart rate (ES <0.2) and only small changes in blood glucose (ES 0.2), which were unrelated to performance. Blood lactate was substantially higher post-TT for CAF and CHO+CAF (ES >0.6).

Conclusion:

After prolonged constant-load cycling, the oral presence of CHO and CAF in chewing gum, independently or in combination, did not improve overall performance but did influence pacing.