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Louise M. Burke, Bronwen Lundy, Ida L. Fahrenholtz and Anna K. Melin

.4 ± 0.4 years 3 × 4 days dietary record using smart phone photography Activity logs and HR. EEE (kcal/kg/min) = (5.95 HRaS × 0.23 age)  − 134/4,186.8 Exercise – – – EEE not adjusted for SA FFM determined by skinfold measures Leptin, ghrelin, and cortisol saliva measured Baseline: 105 Midpoint: 93 End

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D. Enette Larson-Meyer, Kathleen Woolf and Louise Burke

the most frequently sampled tissues, but specific types of white blood cells, urine, saliva, and hair are also used. Serum and plasma concentrations of nutrients tend to reflect recent dietary intake or acute status unless the nutrient is homeostatically regulated (e.g., calcium or sodium) or buffered