This study investigated the effect of caffeine ingestion on neutrophil oxidative burst responses to prolonged cycling. In a two part study, 19 endurance trained male cyclists (Part A – 11; Part B – 8) performed 90 min of exercise at 70% VO2max 1 h after ingesting 6 mg/kg body mass of caffeine (CAF) or placebo (PLA). CAF ingestion had no effect on the PMA-stimulated oxidative burst response (Part A), yet it attenuated the exercise-induced decline in f-MLP stimulated response that occurred with PLA (Part B). CAF ingestion significantly increased serum caffeine concentration and plasma adrenaline concentration following exercise. In addition, circulating lymphocyte count was increased following CAF ingestion whereas there was no effect on neutrophil number. Therefore, although CAF ingestion was associated with an increase in adrenaline, this was not associated with an expected decrease in neutrophil function. This suggests that in the present study, CAF ingestion influenced neutrophil function via alternative mechanisms.
Gary J. Walker, Phillipa Caudwell, Natalie Dixon, and Nicolette C. Bishop
Paula J. Robson, Patrick J.D. Bouic, and Kathryn H. Myburgh
The influence of an antioxidant vitamin supplement on immune cell response to prolonged exercise was determined using a randomized, double-blind, placebo-controlled, cross-over study. Twelve healthy endurance subjects (n = 6 male, n = 6 female; mean ± SD for age, 30.1 ± 6.2 yr; height, 1.76 ± 7 m; body mass, 72.2 ± 10.2 kg; VO2max, 63.7 ± 12 ml · kg–1 · min–1) participated in the study. Following a 3-week period during which subjects ingested a multivitamin and -mineral complex sufficient to meet the recommended daily allowance, they took either a placebo or an antioxidant vitamin supplement (containing 18 mg β-carotene, 900 mg vitamin C, and 90 mg vitamin E) for 7 days prior to a 2-h treadmill run at 65% VO2max. Blood samples were drawn prior to and immediately following exercise. These were analyzed for neutrophil oxidative burst activity, cortisol and glucose concentrations, and white blood cell counts, as well as serum anti-oxidant vitamin concentrations. Plasma vitamin C, vitamin E, and β-carotene concentrations significantly increased following 7-day supplementation (p < .05). In comparison to the placebo group, neutrophil oxidative burst was significantly higher following exercise (p < .05), but no differences were found in any other parameter following the 7-day supplementation period. Although the impact of exercise on neutrophil function is multifactorial, our data suggest that antioxidant supplementation may be of benefit to endurance athletes for the maintenance of this particular function of the innate immune system following the 7-day supplementation period.
Neil P. Walsh, Andrew K. Blannin, Nicolette C. Bishop, Paula J. Robson, and Michael Gleeson
Recent studies have shown that neutrophils can utilize glutamine and that glutamine supplementation can improve neutrophil function in postoperative and burn patients. The present study investigated the influence of oral glutamine supplementation on stimulated neutrophil degranulation and oxidative burst activity following prolonged exercise. Subjects, 7 well-trained men, reported to the laboratory following an overnight fast and cycled for 2 hrs at 60%
Manuela Konrad, David C. Nieman, Dru A. Henson, Krista M. Kennerly, Fuxia Jin, and Sandra J. Wallner-Liebmann
This study tested the acute anti-inflammatory and immune-modulating influence of a quercetin-based supplement consumed by endurance athletes 15 min before an intense 2-hr run. In this randomized, crossover study, 20 runners (11 men, 9 women, age 38.4 ± 2.1 yr) completed two 2-hr treadmill runs at 70% VO2max (3 wk apart). Subjects ingested either 4 quercetin-based chews (Q-chew) or placebo chews (PL) 15 min before the runs. The 4 Q-chews provided 1,000 mg quercetin, 120 mg epigallocatechin 3-gallate, 400 mg isoquercetin, 400 mg each eicosapentaenoic acid and docosahexaenoic acid, 1,000 mg vitamin C, and 40 mg niacinamide. Subjects provided blood samples 30 min before, immediately after, and 1 hr postexercise and were analyzed for plasma quercetin, total blood leukocytes (WBC), C-reactive protein (CRP), 9 cytokines (IL-6, TNFα, GM-CSF, IFNγ, IL-1β, IL-2, IL-8, IL-10, and IL-12p70), granulocyte (GR) and monocyte (MO) phagocytosis (PHAG), and oxidative-burst activity (OBA). Plasma quercetin increased from 80.0 ± 26.0 μg/L to 6,337 ± 414 μg/L immediately postexercise and 4,324 ± 310 μg/L 1 hr postexercise after ingestion of Q-chews, compared with no change in PL (p < .001). Exercise caused significant increases in, CRP, GM-CSF, IL-10, IL-1β, IL-2, IL-6, IL-8, TNFα, GR-PHAG, and MO-PHAG and decreases in GR-OBA and MO-OBA, but no differences in the pattern of change were measured between Q-chew and PL trials. Acute ingestion of Q-chews 15 min before heavy exertion caused a strong increase in plasma quercetin levels but did not counter postexercise inflammation or immune changes relative to placebo.
Michael A. Penkman, Catherine J. Field, Christopher M. Sellar, Vicki J. Harber, and Gordon J. Bell
This study determined the effect of dehydration and rehydration (DR) on performance, immune cell response, and tympanic temperature after high-intensity rowing exercis.
Seven oarswomen completed two simulated 2000-m rowing race trials separated by 72 h in a random, cross-over design. One trial was completed in a euhydrated (E) condition and the other using a DR protocol.
The DR condition resulted in a 3.33 ± 0.14% reduction in body mass (P < .05) over a 24-h period followed by a 2-h rehydration period immediately before the simulated rowing race. There was a greater change in tympanic temperature observed in the DR trial (P < .05). There were increases in the blood concentration of leukocytes, lymphocytes, lymphocyte subsets (CD3+, CD3+/4+, CD3+/8+, CD3−/16+, CD4+/25+; P < .05) and decreases in lymphocyte proliferation and neutrophil oxidative burst activity immediately following the simulated race (P < .05) in both trials. Blood leukocyte and neutrophil concentrations were greater after exercise in the DR trial (P < .05). Whereas most immune measures returned to resting values after 60 min of recovery in both trials, lymphocyte proliferation and the concentrations of CD3+/4+ and CD4+/25+ cells were significantly lower than before exercise. Blood leukocyte and neutrophil concentrations were significantly higher before and after exercise in the E trial.
The effects of dehydration/rehydration did not negatively influence simulated 2000-m rowing race performance in lightweight oarswomen but did produce a higher tympanic temperature and had a differential effect on blood leukocyte, neutrophil, and natural killer (CD3−/16+) cell concentrations after exercise compared with the euhydrated state.
Jose Morales, Carla Ubasart, Mónica Solana-Tramunt, Israel Villarrasa-Sapiña, Luis-Millán González, David Fukuda, and Emerson Franchini
of elite amateur boxers . J Athl Train . 2013 ; 48 ( 1 ): 109 – 117 . PubMed ID: 23672332 doi:10.4085/1062-6050-48.1.05 10.4085/1062-6050-48.1.05 23672332 6. Suzuki M , Nakaji S , Umeda T , et al . Effects of weight reduction on neutrophil phagocytic activity and oxidative burst
Ricardo J.S. Costa, Vera Camões-Costa, Rhiannon M.J. Snipe, David Dixon, Isabella Russo, and Zoya Huschtscha
.g., elastase degranulation and oxidative burst) may play a role in initiating tissue repair ( Peake, 2002 ; Peake et al., 2017 ; Walsh et al., 2011 ), which equate to exercise recovery process similarities. However, certain aspects of neutrophil function are consistently shown to be depressed for several hours after