Search Results

You are looking at 1 - 6 of 6 items for :

  • "peripheral blood mononuclear cells" x
Clear All
Restricted access

David C. Nieman, Giuseppe Valacchi, Laurel M. Wentz, Francesca Ferrara, Alessandra Pecorelli, Brittany Woodby, Camila A. Sakaguchi and Andrew Simonson

then were allowed to leave the lab. The participants engaged in a 2-week washout period without supplements, crossed over, and then repeated all of the procedures. Peripheral Blood Mononuclear Cell and Plasma Isolation Blood samples were collected in heparinized tubes and processed within 30 min after

Restricted access

Marcia A. Chan, Alexander J. Koch, Stephen H. Benedict and Jeffrey A. Potteiger

The effect of carbohydrate supplementation (CHO) on interleukin 2 (IL-2) and interleukin 5 (IL-5) secretion following acute resistance exercise was examined in 9 resistance-trained males. Subjects completed a randomized, double-blind protocol with exercise separated by 14 days. The exercise consisted of a high intensity, short rest interval squat workout. Subjects consumed 1.0 g · kg body mass-1 CHO or an equal volume of placebo (PLC) 10 min prior to and 10 min following exercise. Blood was collected at rest (REST), immediately post exercise (POST), and at 1.5 h of recovery (1.5 h POST). Isolated peripheral blood mononuclear cells were stimulated with PHA and assayed for IL-2 and IL-5 secretion. IL-2 secretion was significantly decreased at POST for both the PLC and CHO groups. However, the degree of decrease was less in the CHO group (16%) than in the PLC group (48%), and this difference was statistically significant. These responses were transient, and the values returned to normal by 1.5 h POST. A mild and transient but significant decrease in IL-5 secretion by the PLC group was observed at POST (26%) compared to REST. No significant decrease was observed in IL-5 secretion for CHO from REST to POST (12%). These data support a possible effect of carbohydrate supplementation on IL-2 and IL-5 secretion following high-intensity resistance exercise.

Restricted access

Takeshi Kokubo, Yuta Komano, Ryohei Tsuji, Daisuke Fujiwara, Toshio Fujii and Osamu Kanauchi

Peripheral Blood Mononuclear Cells and FACS Analysis Peripheral blood mononuclear cells were isolated using Ficoll-Paque PREMIUM (GE Healthcare Bio-Sciences, Uppsala, Sweden). Cells were stained for 30 min at 4 °C with fluorescent dye-conjugated Abs as follows: CD86-PE (GL1; BD Pharmingen, San Diego, CA

Full access

Bindu P. Gopalan, Mary Dias, Karthika Arumugam, Reena R. D’Souza, Mathew Perumpil, Prasanna Kulkarni, Udaykumar Ranga and Anita Shet

the plasma LPS concentration (±SD) was observed among exercisers and nonexercisers at Y2 using the limulus amebocyte lysate assay (0.35 [0.12] EU/mL; 0.36 [0.22] EU/mL; P  = .75) CD3 + CD8 + CD38 Expression and Gene Expression Profile Peripheral blood mononuclear cells were used for the analysis of

Restricted access

Livia Victorino Souza, Franciele De Meneck, Vanessa Oliveira, Elisa Mieko Higa, Eliana Hiromi Akamine and Maria do Carmo Franco

with dipotassium ethylenediaminetetraacetic acid were processed within 1 hour after blood collection for isolation of EPCs (16 mL) and assessment of NO and VEGF-A (4 mL). EPCs Quantification by Flow Cytometer and In Vitro Functional Capacity Assay Peripheral blood mononuclear cells (MNCs) were isolated

Restricted access

Kirsty J. Elliott-Sale, Adam S. Tenforde, Allyson L. Parziale, Bryan Holtzman and Kathryn E. Ackerman

Metabolism, 102 ( 5 ), 1413 – 1439 . PubMed ID: 28368518 doi:10.1210/jc.2017-00131 10.1210/jc.2017-00131 Grasso , G. , Lodi , L. , Lupo , C. , & Muscettola , M. ( 1997 ). Glucocorticoid receptors in human peripheral blood mononuclear cells in relation to age and to sport activity . Life Sciences